Effect of triclosan on Salmonella typhimurium at different growth stages and in biofilms.
Effect of triclosan on Salmonella typhimurium at different growth stages and in biofilms.
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DOI：
10.1111/j.1574-6968.2006.00547.x
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发表时间：
2007-02
影响因子：
2.1
通讯作者：
M. Tabak;K. Scher;E. Hartog;U. Romling;K. Matthews;M. Chikindas;S. Yaron
中科院分区：
文献类型：
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作者：
M. Tabak;K. Scher;E. Hartog;U. Romling;K. Matthews;M. Chikindas;S. Yaron
三氯生是一种有效的杀生物剂，包含在各种产品中。本研究的目的是调查三氯生的肠道沙门氏菌和生物膜相关的鼠伤寒沙门氏菌的敏感性，并确定潜在的适应机制。研究了三氯生对嗜盐沙门氏菌（对数期和稳定期）、生物膜相关细胞、生物膜破裂产生的细菌和生物膜缺陷突变体的影响。1000 μ g mL（-1）三氯生在10分钟内观察到指数生长细胞减少8个对数，静止细胞减少3.6个对数，生物膜缺陷突变体的静止细胞减少6.3个对数（P<0.05）。生物膜相关细胞耐受（1-log减少）。然而，生物膜衍生的细胞表现出类似于静止期细胞的三氯生敏感性。三氯生诱导fabI和micF的转录。在生物膜内，三氯生还上调acrAB的转录，编码外排泵marA和纤维素合成编码基因bcsA和bcsE。因此，生物膜内的沙门氏菌可以经历减少的流入，增加的流出和增强的胞外多糖产生。我们的研究结果表明，沙门氏菌对三氯生在生物膜中的耐受性归因于通过细胞外基质的低扩散，而基因表达的变化可能会提供进一步的耐药性三氯生和其他抗菌剂。
Triclosan is a potent biocide that is included in a diverse range of products. This research was aimed to investigate the susceptibility of planktonic and biofilm-associated Salmonella enterica serovar Typhimurium to triclosan, and to identify potential mechanisms of adaptation. The effect of triclosan was studied on planktonic Salmonella (log and stationary phases), on biofilm-associated cells, on bacteria derived from disrupted biofilms and on a biofilm-deficient mutant. An eight-log reduction of exponentially growing cells was observed with 1000 micro g mL(-1) triclosan within 10 min, a 3.6-log reduction in stationary cells and a 6.3-log reduction in stationary cells of a biofilm-deficient mutant (P<0.05). Biofilm-associated cells were tolerant (1-log reduction). However, biofilm-derived cells showed sensitivity to triclosan similar to stationary-phase cells. Triclosan induced the transcription of fabI and micF. Within biofilms, triclosan also up-regulated the transcription of acrAB, encoding for an efflux pump, marA, and the cellulose-synthesis-coding genes bcsA and bcsE. Thus, Salmonella within biofilms could experience reduced influx, increased efflux and enhanced exopolysaccharides production. Our results demonstrated that the tolerance of Salmonella towards triclosan in the biofilm was attributed to low diffusion through the extracellular matrix, while changes of gene expression might provide further resistance to triclosan and to other antimicrobials.