Identification of Chicken Pulmonary miRNAs Targeting PB1, PB1-F2, and N40 Genes of Highly Pathogenic Avian Influenza Virus H5N1 In Silico.
Identification of Chicken Pulmonary miRNAs Targeting PB1, PB1-F2, and N40 Genes of Highly Pathogenic Avian Influenza Virus H5N1 In Silico.
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DOI：
10.4137/bbi.s14631
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发表时间：
2014
影响因子：
5.8
通讯作者：
Mishra A
中科院分区：
文献类型：
--
作者：
Kumar A;Vn MA;Raut AA;Sood R;Mishra A
高致病性禽流感（HPAI）是由正粘病毒科a型禽流感病毒引起的一种法定病毒性疾病。A型流感基因组由8段负义RNA组成。RNA片段2编码PB1、PB1- f2和N40三种蛋白，它们是通过核糖体渗漏扫描和再起始从相同的mRNA翻译而来。由于这些蛋白对病毒复制和发病机制至关重要，靶向它们的表达可能是控制和抵抗高致病性禽流感的方法之一。MicroRNAs是一种短的非编码rna，可调节多种生物过程，如细胞生长、组织分化、细胞凋亡和病毒感染。本研究利用异源双工的热力学稳定性、种子序列的互补性、靶序列的保守性和靶点可及性等不同的筛选参数，对鸡肺中表达的300个microrna进行了HPAI病毒（H5N1） 2片段转录物的筛选，以鉴定可能靶向H5N1 2片段转录物的microrna。鸡miRNAs gga-mir-133c、gga-mir-1710和gga-mir-146c*被预测可靶向PB1、PB1- f2和N40蛋白的表达。这表明鸡具有抵抗/耐受H5N1感染的遗传潜力，可以适当地利用这些潜力设计控制鸡禽流感的策略。
Highly pathogenic Avian influenza (HPAI) is a notifiable viral disease caused by avian influenza type A viruses of the Orthomyxoviridae family. Type A influenza genome consists of eight segments of negative-sense RNA. RNA segment 2 encodes three proteins, PB1, PB1-F2, and N40, which are translated from the same mRNA by ribosomal leaky scanning and reinitiation. Since these proteins are critical for viral replication and pathogenesis, targeting their expression can be one of the approaches to control and resist HPAI. MicroRNAs are short noncoding RNAs that regulate a variety of biological processes such as cell growth, tissue differentiation, apoptosis, and viral infection. In this study, a set of 300 miRNAs expressed in chicken lungs were screened against the HPAI virus (H5N1) segment 2 with different screening parameter like thermodynamic stability of heteroduplex, seed sequence complementarity, conserved target sequence, and target-site accessibility for identifying miRNAs that can potentially target the transcript of segment 2 of H5N1. Chicken miRNAs gga-mir-133c, gga-mir-1710, and gga-mir-146c* are predicted to target the expression of PB1, PB1-F2, and N40 proteins. This indicates that chicken has genetic potential to resist/tolerate H5N1 infection and these can be suitably exploited in designing strategies for control of avian influenza in chicken.