Effects of non-steroidal antiinflammatory drugs on D-serine-induced oxidative stress in vitro
Effects of non-steroidal antiinflammatory drugs on D-serine-induced oxidative stress in vitro
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DOI：
10.3109/01480545.2011.633086
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发表时间：
2012-10-01
影响因子：
2.6
通讯作者：
Yalcin, Ayfer
中科院分区：
文献类型：
--
作者：
Armagan, Guliz;Kanit, Lutfiye;Yalcin, Ayfer
炎症对再生能力降低的器官（如大脑）是有害的。近年来，非甾体类抗炎药（NSAID）在阿尔茨海默病、帕金森病、亨廷顿病和多发性硬化症中的治疗作用成为研究热点。兴奋性毒性是当兴奋性神经递质谷氨酸的受体（例如N-甲基-D-天冬氨酸（NMDA））过度活化时的病理过程。这一过程可能与神经退行性疾病有关。D-丝氨酸是NMDA受体的促凝剂之一，并且D-丝氨酸水平升高与兴奋性毒性相关。在我们的研究中，甲芬那酸，对乙酰氨基酚，和萘普生钠的潜在神经保护作用进行了研究，对D-丝氨酸诱导的氧化应激在体外大鼠脑。为了显示其潜在的神经保护特性，将NSAID与D-丝氨酸孵育，并测量不同处理后脑的活性氧（ROS）、丙二醛和蛋白质羰基含量。我们的研究结果表明，在本研究中使用的非甾体抗炎药显着减少ROS的产生，脂质过氧化和蛋白质氧化对D-丝氨酸治疗。
Inflammation is deleterious for organs with reduced capacity of regeneration, such as the brain. Recently, studies have focused on investigating the therapeutic effects of nonsteroidal anti-inflammatory drugs (NSAIDs) in Alzheimer's disease, Parkinson's disease, Huntington's disease, and multiple sclerosis. Excitotoxicity is the pathological process when receptors for the excitatory neurotransmitter glutamate, such as the N-methyl-D-aspartate (NMDA), receptors are overactivated. This process may be involved in neurodegenerative diseases. D-serine is one of the coagonist of NMDA receptors, and increased levels of D-serine are associated with excitotoxicity. In our study, the potential neuroprotective effects of mefenamic acid, acetaminophen, and naproxen sodium were investigated against D-serine-induced oxidative stress in the rat brain in vitro. To show their potential neuroprotective properties, NSAIDs were incubated with D-serine and reactive oxygen species (ROS), malondialdehyde, and protein carbonyl content of the brain after different treatments were measured. Our results demostrate that NSAIDs used in the present study significantly reduced ROS production, lipid peroxidation, and protein oxidation against D-serine treatment.