HLA-DR- and CD11c-positive dendritic cells penetrate beyond well-developed epithelial tight junctions in human nasal mucosa of allergic rhinitis
HLA-DR- and CD11c-positive dendritic cells penetrate beyond well-developed epithelial tight junctions in human nasal mucosa of allergic rhinitis
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DOI：
10.1369/jhc.4a6539.2005
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发表时间：
2005-05-01
影响因子：
3.2
通讯作者：
Sawada, N
中科院分区：
文献类型：
--
作者：
Takano, K;Kojima, T;Sawada, N
上呼吸道上皮屏障在宿主防御中起着至关重要的作用。本研究通过观察变应性鼻炎患者鼻粘膜上皮细胞紧密连接的表达和功能，探讨变应性鼻炎中是否存在树突状细胞（DCs）对抗原的监控作用。在逆转录-聚合酶链反应中，在鼻粘膜中检测到紧密连接蛋白occludin、JAM-1、ZO-1和claudin-1、-4、-7、-8、-12、-13和-14的mRNA。封闭蛋白、JAM-1和ZO-1共定位于鼻粘膜假复层上皮的最上层，而封闭蛋白-1、-4和-7被发现遍及上皮。在鼻粘膜的冷冻断裂复制品中，连续的紧密连接链形成发育良好的网络。通过染料示踪剂测量的上皮屏障功能在鼻粘膜上皮中的闭合蛋白阳性紧密连接中保持良好。HLA-DR和CD 11 c阳性DC表达claudin-1，并在变应性鼻炎鼻粘膜上皮中渗透超过occludin。这些结果表明，DC可以很容易地访问抗原超越上皮紧密连接在人鼻粘膜变应性鼻炎。
The epithelial barrier of the upper respiratory tract plays a crucial role in host defense. In this study, to elucidate whether there is antigen monitoring by dendritic cells (DCs) beyond the epithelial tight-junction barrier in allergic rhinitis, we investigated the expression and function of tight junctions and characterized DCs in the epithelium of nasal mucosa from patients with allergic rhinitis. In reverse transcription-polymerase chain reaction, mRNAs of tight-junction proteins occludin, JAM-1, ZO-1, and claudin-1, -4, -7, -8, -12, -13, and -14 were detected in the nasal mucosa. Occludin, JAM-1, and ZO-1 were colocalized in the uppermost layer in the pseudostratified epithelium of the nasal mucosa, whereas claudin-1, -4, and -7 were found throughout the epithelium. in freeze-fracture replicas of the nasal mucosa, continuous tight-junction strands formed well-developed networks. Epithelial barrier function measured by a dye tracer was well maintained in occludin-positive tight junctions in the epithelium of the nasal mucosa. HLA-DR- and CD11c-positive DCs expressed claudin-1 and penetrated beyond occludin in the epithelium of the nasal mucosa with, but not without, allergic rhinitis. These results indicate that DCs may easily access antigens beyond epithelial tight junctions in the human nasal mucosa of allergic rhinitis.