Antibacterial properties and human gingival fibroblast cell compatibility of TiO2/Ag compound coatings and ZnO films on titanium-based material
Antibacterial properties and human gingival fibroblast cell compatibility of TiO2/Ag compound coatings and ZnO films on titanium-based material
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DOI：
10.1007/s00784-010-0504-9
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发表时间：
2012-02-01
影响因子：
3.4
通讯作者：
Huang, Heng-Li
中科院分区：
文献类型：
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作者：
Chang, Yin-Yu;Lai, Chih-Ho;Huang, Heng-Li
钛基材料广泛应用于生物医学植入物组件，并成功应用于各种类型的骨锚定重建。然而，在牙科植入物中，钛材料不仅接触骨，而且接触牙龈组织，并且部分暴露于包含细菌的口腔。本研究使用二氧化钛和银（TiO 2/Ag）复合涂层和氧化锌（ZnO）薄膜来增强钛基植入物的抗菌活性。通过测量接触角来检查每个样品的疏水性。在涂层上培养变形链球菌和人牙龈成纤维细胞（HGF），采用Syto 9荧光染色法和MTT法测定涂层的抗菌效果和细胞相容性。对于TiO 2/Ag样品，在较高的功率下在板上沉积Ag（这增加了Ag的比例）增加了接触角和疏水性。50 W TiO 2/Ag样品的细菌计数最低，其含有5.9%的Ag。ZnO样品的接触角没有表现出相同的趋势。ZnO涂覆的样品上的抗菌效果更高，因为与对照样品（Ti板）相比，ZnO样品上的细菌计数低三倍。从MTT分析测试，平均光密度值的TiO 2/Ag涂覆的样品后72小时的HGF粘附是从未涂覆的Ti获得的值相似。然而，生物相容性低于ZnO薄膜比对照样品。ZnO薄膜的抗菌活性高于TiO 2/Ag涂层，但细胞相容性低于TiO 2/Ag涂层。
Titanium (Ti)-based materials are widely used in biomedical implant components and are applied successfully in various types of bone-anchored reconstructions. However, in dental implants the Ti materials contact not only bone but also gingival tissues, and are partially exposed to the oral cavity that includes bacteria. This study used titania and silver (TiO2/Ag) compound coatings and zinc oxide (ZnO) films to enhance the antibacterial activity of the Ti-based implant. The hydrophobicity of each sample was examined by measuring the contact angle. Streptococcus mutans and human gingival fibroblast (HGF) was cultured on the coated samples, and the antibacterial effects and cell compatibility were determined using a Syto9 fluorescence staining and MTT methods. For the TiO2/Ag samples, depositing Ag on the plate at a higher power (which increased the proportion of Ag) increased the contact angle and the hydrophobicity. The bacterial count was lowest for the 50 W TiO2/Ag sample, which contained 5.9% Ag. The contact angles of the ZnO samples did not show the same tendency. The antibacterial effect was higher on ZnO-coated samples since bacterial count was threefold lower on ZnO samples as compared to control samples (Ti plate). From the MTT assay test, the mean optical density values for TiO2/Ag-coated samples after 72 h of HGF adhesion were similar to the value obtained from the uncoated Ti. However, biocompatibility was lower on ZnO films than in control samples. Conclusively, the antibacterial activity was higher but the cell compatibility was lower on ZnO films than on TiO2/Ag coatings.