Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible involvement in ovulation
Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible involvement in ovulation
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DOI：
10.1016/j.mce.2010.09.015
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发表时间：
2011-01-30
影响因子：
4.1
通讯作者：
Takahashi, Takayuki
中科院分区：
文献类型：
--
作者：
Fujimori, Chika;Ogiwara, Katsueki;Takahashi, Takayuki
成熟的medaka卵巢卵泡的体外排卵被环氧化酶（COX）抑制剂或前列腺素E-2受体（EP）拮抗剂抑制。在三个medaka COX基因中，ptgs2在鱼类卵巢中表达最多。在所有大小的生长卵泡中均检测到ptgs2转录本。在24小时的产卵周期中，大卵泡含有相当稳定的ptgs2 mRNA。在产卵周期中，大卵泡的COX酶活性和前列腺素E-2水平也保持不变。随着排卵时间的临近，大卵泡中前列腺素E-2受体EP4b (ptger419) mRNA的表达急剧上调。目前的研究结果表明前列腺素E-2。它可能由COX-2产生，参与medaka的排卵，并且EP4b可能是在此过程中负责发挥前列腺素E-2作用的受体。2010爱思唯尔爱尔兰有限公司版权所有。
In vitro ovulation of mature medaka ovarian follicles was inhibited by inhibitors of cyclooxygenase (COX) or by an antagonist of the prostaglandin E-2 receptor (EP). Of the three medaka COX genes, ptgs2 was most dominantly expressed in the fish ovary. The ptgs2 transcript was detected in all sizes of growing follicles. In a 24-h spawning cycle, large-sized follicles contained ptgs2 mRNA at a fairly constant level. The levels of COX enzyme activity and prostaglandin E-2 were also constant in the large-sized follicles during the spawning cycle. The expression of prostaglandin E-2 receptor EP4b (ptger419) mRNA was drastically upregulated in the large-sized follicles as the ovulation time approached. The current results indicate that prostaglandin E-2. which might be produced by COX-2, is involved in the ovulation of medaka, and that EP4b is likely the receptor responsible for exerting the action of prostaglandin E-2 in the process. (C) 2010 Elsevier Ireland Ltd. All rights reserved.