LEADER SEQUENCES OF MURINE CORONAVIRUS MESSENGER-RNAS CAN BE FREELY REASSORTED - EVIDENCE FOR THE ROLE OF FREE LEADER RNA IN TRANSCRIPTION
LEADER SEQUENCES OF MURINE CORONAVIRUS MESSENGER-RNAS CAN BE FREELY REASSORTED - EVIDENCE FOR THE ROLE OF FREE LEADER RNA IN TRANSCRIPTION
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DOI：
10.1073/pnas.83.12.4204
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发表时间：
1986-06-01
影响因子：
11.1
通讯作者：
LAI, MMC
中科院分区：
文献类型：
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作者：
MAKINO, S;STOHLMAN, SA;LAI, MMC
小鼠肝炎病毒 (MHV) 在受感染细胞的细胞质中复制，在每个病毒特异性 mRNA 的 5 ' 端包含相同的前导 RNA 序列。先前的研究表明，这些 mRNA 的合成不涉及传统的 RNA 剪接，而是可能需要游离前导 RNA 的引发。在本次交流中，我们证明，在两种不同 MHV 的混合感染过程中，可以在共感染病毒的 mRNA 上以高频率检测到来自一种病毒的前导 RNA 序列，就好像前导序列和 mRNA 是由两个随机分离的 RNA 片段连接在一起一样。这一发现表明 MHV 和 mRNA 转录利用具有反式作用特性的独立转录的前导 RNA 种类。因此，这项研究为支持冠状病毒 mRNA 合成的“前导引物转录”独特模型提供了进一步的证据。
Mouse hepatitis viruw (MHV), which replicates in cytoplasm of infected cells, contains an identical leader RNA sequence at the 5'' end of each of the virus-specific mRNAs. Previous studies suggested that the synthesis of these mRNAs does not involve conventional RNA splicing and may instead require priming by a free leader RNA. In this communication, we demonstrate that, during a mixed infection with two different MHVs, the leader RNA sequences from one virus could be detected on the mRNAs of the coinfecting virus at a high frequency, as if the leader sequence and mRNAs were joined together from two randomly segregating RNA segments. This finding demonstrates that MHV and mRNA transcription utilizes independently transcribed leader RNA species that possess the trans-acting property. This study thus provides further evidence in support of the unique model of "leader-primed transcription" for coronavirus mRNA synthesis.