Tandem Mass Spectrometry Analysis Using the ThermoFinnigan LCQ System.
Tandem Mass Spectrometry Analysis Using the ThermoFinnigan LCQ System.
复制标题
使用 ThermoFinnigan LCQ 系统进行串联质谱分析。
DOI：
10.1101/pdb.prot4554
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
L. Hays
中科院分区：
文献类型：
--
作者：
D. Schieltz;M. Washburn;L. Hays
引言本方案为使用ThermoFinnigan LCQ系统进行典型的数据相关MS/MS采集分析的参数设置提供了指导。该方法包括一个连续循环，从一次MS扫描（扫描一）开始，记录梯度中该时刻存在的所有离子的m/z值，然后进行两轮MS/MS。初始MS/MS扫描是从MS扫描记录的第一个最强离子。第二次MS/MS扫描是从第一次扫描记录的第二个最强离子。动态排除被激活以提高分析期间的蛋白质鉴定能力。
INTRODUCTIONThis protocol provides guidance for setting the parameters for a typical data-dependent MS/MS acquisition analysis using the ThermoFinnigan LCQ system. The method consists of a continual cycle beginning with one scan of MS (scan one), which records all of the m/z values of the ions present at that moment in the gradient, followed by two rounds of MS/MS. The initial MS/MS scan is of the first most-intense ion recorded from the MS scan. The second MS/MS scan is of the second most-intense ion recorded from scan one. Dynamic exclusion is activated to improve the protein identification capacity during the analysis.