Molecular cloning of the human Edg2 protein and its identification as a functional cellular receptor for lysophosphatidic acid
Molecular cloning of the human Edg2 protein and its identification as a functional cellular receptor for lysophosphatidic acid
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DOI：
10.1006/bbrc.1997.6150
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发表时间：
1997-02-24
影响因子：
3.1
通讯作者：
Goetzl, EJ
中科院分区：
文献类型：
--
作者：
An, SZ;Dickens, MA;Goetzl, EJ
从人肺cDNA文库中克隆了与绵羊Edg2蛋白同源的基因，全长编码364个氨基酸的蛋白，属于鸟核苷酸结合蛋白偶联受体超家族，人Edg2基因在人类组织中广泛分布，在脑中含量最高，表达人Edg2蛋白的HEK293细胞在血清反应元件报告基因分析中显示对溶血磷脂酸(LPA)的反应增强，与其他溶血磷脂相比，LPA具有浓度依赖性和特异性，人Edg2在CHO细胞中的过度表达与[H-3]-LPA特异性结合的增加有关。最近，小鼠的Edg2蛋白也被鉴定为LPA的受体(Hecht等人，1996，J.Cell Biol，135,1071)，因此，我们得出结论，人的Edg2蛋白是LPA的细胞受体。(C)1997年学术出版社。
A cDNA homologous to that encoding sheep Edg2 protein was cloned from a human lung cDNA library, The full-length sequence encodes a 364-amino acid protein which belongs to the superfamily of guanine nucleotide-binding (G) protein-coupled receptors, Human Edg2 mRNA is widely distributed in human tissues with the highest abundance in brain, HEK293 cells expressing the human Edg2 protein showed an elevated response to lysophosphatidic acid (LPA) in a serum response element reporter gene assay, which was LPA concentration-dependent and specific to LPA compared to other lysophospholipids, Over-expression of human Edg2 in CHO cells correlated with increases in specific binding of [H-3]-LPA. Recently, the mouse counterpart of Edg2 protein also was identified as a receptor for LPA (Hecht et al., 1996, J. Cell Biol, 135, 1071), Therefore, it is concluded that the human Edg2 protein functions as a cellular receptor for LPA. (C) 1997 Academic Press.