Novel Drosophila laminin A chain reveals structural relationships between laminin subunits.
Novel Drosophila laminin A chain reveals structural relationships between laminin subunits.
复制标题
新型果蝇层粘连蛋白 A 链揭示了层粘连蛋白亚基之间的结构关系。
DOI：
10.1096/fasebj.7.2.8440414
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Fessler,JH
中科院分区：
文献类型：
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作者：
MacKrell,AJ;Kusche-Gullberg,M;Garrison,K;Fessler,JH
层粘连蛋白是由 A、B1 和 B2 链组成的三聚体糖蛋白，在细胞粘附和分化以及基底膜组装中发挥重要作用。有证据表明，多个独立的基因产物可用于提供 A、B1 和 B2 样链，从而产生不同的层粘连蛋白分子。 A 链样多肽组是最有差异的。每条链的氨基末端部分称为短臂，由交替的富含半胱氨酸和球状结构域组成。新表征的果蝇拉敏蛋白 A 链的氨基末端三分之二似乎编码一种新的结构，但此处提出的分析表明，其氨基酸序列的大部分与其他 A、B1 和 B2 链中发现的序列相关。果蝇A链的一部分由类似于B2链中发现的序列组成。这些序列与脊椎动物 A 链的一部分平行，表明存在共享祖先 A 链的结构。然而，与脊椎动物的 A 链不同，果蝇 A 链还包含 B1 链的典型序列，这表明它的基因一定是通过来自不同原始层粘连蛋白基因的片段重组而产生的。库什-古尔伯格，M.；加里森，K.； Fessler, J. H. NovelDrosophilialaminin A 链揭示了层粘连蛋白亚基之间的结构关系。FASEB J.7: 375‐381； 1993年。
Laminins are trimeric glycoproteins composed of A, B1, and B2 chains that play important roles in cell adhesion and differentiation and in the assembly of basement membranes. There is evidence that multiple independent gene products can be used to supply the A, B1, and B2‐like chains, thereby generating diverse laminin molecules. The set of A chain‐like polypeptides are the most divergent. The amino‐terminal portion of each chain, called the short arm, is composed of alternating cysteine‐rich and globular domains. The amino‐terminal two‐thirds of the newly characterizedDrosophilalaminin A chain appears to encode a novel structure, but the analysis presented here shows that substantial portions of its amino acid sequence are related to sequences found in other A, B1, and B2 chains. A portion of theDrosophilaA chain is composed of sequences like those found in B2 chains. These sequences parallel a portion of the vertebrate A chains, suggesting a structure for a shared ancestral A chain. However, unlike vertebrate A chains, theDrosophilaA chain also contains sequences typical of B1 chains, suggesting that its gene must have arisen by recombination of segments from different primordial laminin genes.— MacKrell, A. J.; Kusche‐Gullberg, M.; Garrison, K.; Fessler, J. H. NovelDrosophilialaminin A chain reveals structural relationships between laminin subunits.FASEB J.7: 375‐381; 1993.