Persistence of Methanosaeta populations in anaerobic digestion during process instability
Persistence of Methanosaeta populations in anaerobic digestion during process instability
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DOI：
10.1007/s10295-015-1632-7
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发表时间：
2015-08-01
影响因子：
3.4
通讯作者：
He, Qiang
中科院分区：
文献类型：
--
作者：
Chen, Si;He, Qiang
厌氧消化是一种处理有机废物和生产沼气的可持续技术。在厌氧消化过程中，破醋酸产甲烷占甲烷产量的大部分。因此，维持稳健的破乙酸产甲烷菌对于稳定的工艺性能是重要的。由于在高乙酸盐浓度下更快的生长动力学，已经认为甲烷八叠球菌在经常经历高乙酸盐水平的不稳定厌氧消化过程中比甲烷八叠球菌更普遍。在多个连续厌氧消化器中监测产甲烷菌群体动态500天。从定量聚合酶链反应分析的结果表明，甲烷八叠球菌占主导地位的甲烷八叠球菌在高乙酸水平高达44 mM的厌氧消化，这表明甲烷八叠球菌作为一个强大的和有效的乙酸破碎的候选人弹性厌氧甲烷转化的潜力。需要进一步的努力，以确定机制，这些产甲烷菌在本研究中观察到的高乙酸水平的意外竞争力。
Anaerobic digestion is a sustainable technology for the treatment of organic waste and production of biogas. Acetoclastic methanogenesis accounts for the majority of methane production in anaerobic digestion. Therefore, sustaining robust acetoclastic methanogens is important for stable process performance. Due to faster growth kinetics at high acetate concentrations, it has been considered that Methanosarcina would be more prevalent than Methanosaeta in unstable anaerobic digestion processes which frequently experience high acetate levels. Methanogen population dynamics were monitored in multiple continuous anaerobic digesters for 500 days. Results from quantitative polymerase chain reaction analysis show that Methanosaeta dominated over Methanosarcina in anaerobic digestion at high acetate levels up to 44 mM, suggesting the potential of Methanosaeta as a robust and efficient acetoclastic candidate for resilient anaerobic methane conversion. Further efforts are needed to identify mechanisms contributing to the unexpected competitiveness of these methanogens at high acetate levels observed in this study.