Nitric oxide and thioredoxin type 1 modulate the activity of caspase 8 in HepG2 cells.
Nitric oxide and thioredoxin type 1 modulate the activity of caspase 8 in HepG2 cells.
复制标题
一氧化氮和 1 型硫氧还蛋白调节 HepG2 细胞中 caspase 8 的活性。
DOI：
10.1016/j.bbrc.2009.12.036
复制
发表时间：
2010
影响因子：
3.1
通讯作者：
Stoyanovsky,DetchoA
中科院分区：
文献类型：
--
作者：
Sengupta,Rajib;Billiar,TimothyR;Kagan,ValerianE;Stoyanovsky,DetchoA
在此，我们报道了一氧化氮（NO）和硫氧还蛋白/硫氧还蛋白还原酶系统影响HepG2细胞中caspase 8的活性。细胞暴露于NO导致caspase 8的抑制，而随后细胞在无NO培养基中孵育导致蛋白酶的自发再激活。后一过程在硫氧还蛋白还原酶缺乏的HepG2细胞中被抑制，然而，硫辛酸显著地重新激活了caspase 8。这些数据表明，在caspase 3诱导蛋白水解损伤之前，外源性细胞凋亡可受到氧化还原调节。
Herein, we report that nitric oxide (NO) and the thioredoxin/thioredoxin reductase system affect the activity of caspase 8 in HepG2 cells. Exposure of cells to NO resulted in inhibition of caspase 8, while a subsequent incubation of the cells in NO-free medium resulted in spontaneous reactivation of the protease. The latter process was inhibited in thioredoxin reductase-deficient HepG2 cells, in which, however, lipoic acid markedly reactivated caspase 8. The data obtained suggest that extrinsic apoptosis can be subjected to redox regulation before induction of proteolytic damage by caspase 3.