On the location of linker DNA in the chromatin fiber. Studies with immobilized and soluble micrococcal nuclease.
On the location of linker DNA in the chromatin fiber. Studies with immobilized and soluble micrococcal nuclease.
复制标题
关于连接 DNA 在染色质纤维中的位置。
DOI：
10.1006/jmbi.1994.1045
复制
发表时间：
1994
影响因子：
5.6
通讯作者：
vanHolde,K
中科院分区：
文献类型：
--
作者：
Leuba,SH;Zlatanova,J;vanHolde,K
鸡红细胞染色质纤维的结构已探测使用微球菌核酸酶，膜固定和游离在溶液中。在所使用的极其温和的消化条件下，接头DNA几乎完全被保护免于在30 nm纤维中被固定化酶或游离酶消化，而在更延伸的结构中它是容易接近的。用戊二醛固定的染色质纤维进行的对照实验得到了基本相同的结果。用中等缩合度的纤维进行的实验揭示了紧密度与接头DNA对消化的抗性之间的直接关系。我们的研究结果有利于模型，其中访问的链接器是有限的局部空间位阻，由于高度压缩，而不是在纤维的中心内化。
The structure of chicken erythrocyte chromatin fibers has been probed using micrococcal nuclease, both membrane-immobilized and free in solution. Under the extremely mild digestion conditions used, the linker DNA is almost completely protected against digestion with either immobilized or free enzyme in the 30 nm fibers, whereas it is readily accessible in the more extended structures. Control experiments with glutaraldehyde-fixed chromatin fibers gave essentially the same results. Experiments with fibers of intermediate degree of condensation revealed a direct relationship between the degree of compaction and the resistance of linker DNA to digestion. Our results favor models in which access to the linkers is limited by local steric hindrance due to the high compaction, rather than by internalization in the center of the fibers.