Proinsulin content of pancreas in human fetuses of healthy mothers.
Proinsulin content of pancreas in human fetuses of healthy mothers.
复制标题
健康母亲的人类胎儿胰腺的胰岛素原含量。
DOI：
10.1016/s0140-6736(70)90522-2
复制
发表时间：
1970
期刊：
影响因子：
168.9
通讯作者：
T. Fraser
中科院分区：
文献类型：
--
作者：
G. Rastogi;J. Letarte;T. Fraser
用SephadexG-50'从人胎胰腺提取物中分离出的胰岛素原占总免疫反应性胰岛素（I.R.I.）无论胎儿的年龄如何。在离体大鼠附睾脂肪细胞上，人胰岛素原在其I.R.I.的约20%时具有胰岛素样活性。经部分胰蛋白酶消化后，其胰岛素免疫反应性增强40%，生物活性增强800%以上。
Proinsulin separated from human fetal pancreatic extracts using Sephadex G-50' comprised 0.26-10.6% of the total immunoreactive insulin (I.R.I.) values irrespective of the age of the fetus. On the isolated rat epididymal fat cells, human proinsulin had insulin-like activity at about 20% of its I.R.I. value; and after partial tryptic digestion, its insulin immunoreactivity was enhanced by 40% and its biological activity by over 800%.