Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.
Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.
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某些人类肿瘤细胞产生的转化生长因子：与表皮生长因子受体相互作用的多肽。
DOI：
10.1073/pnas.77.9.5258
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发表时间：
1980
影响因子：
11.1
通讯作者：
J. D. Larco
中科院分区：
文献类型：
--
作者：
G. Todaro;C. Fryling;J. D. Larco
培养中的三种不同的人类肿瘤系（横纹肌肉瘤、支气管癌和转移性黑色素瘤）将蛋白质（转化生长因子，TGF）释放到培养基中，从而赋予未转化的成纤维细胞转化的表型。这些蛋白质对酸和热稳定；在大鼠和人类成纤维细胞中产生深刻的形态变化；并使正常的贴壁依赖性细胞能够在琼脂中生长。转化蛋白的去除导致细胞表型的逆转。主要活性与表皮生长因子 (EGF) 细胞膜受体相互作用。来自这些肿瘤细胞的肽的作用与鼠肉瘤病毒转化的啮齿动物细胞释放的肉瘤生长因子（SGF）相似。最不依赖贴壁的肿瘤细胞释放最多的 TGF。在含有大量游离 EGF 膜受体的细胞（正常人成纤维细胞和人癌）培养液中未检测到 EGF 相关 TGF。
Three different human tumor lines in culture, a rhabdomyosarcoma, a bronchogenic carcinoma and a metastatic melanoma, release proteins (transforming growth factors, TGFs) into the medium that confer the transformed phenotype on untransformed fibroblasts. These proteins are acid and heat-stable; produce profound morphologic changes in rat and human fibroblasts; and enable normal anchorage-dependent cells to grow in agar. Removal of the transforming protein results in a reversion of cell phenotype. The major activity interacts with epidermal growth factor (EGF) cell membrane receptors. The peptides from these tumor cells are similar in their action to the sarcoma growth factor (SGF) released by murine sarcoma virus-transformed rodent cells. The most anchorage-independent tumor cells released the most TGFs. EGF-related TGFs were not detectable in fluids from cultures of cells with high numbers of free EGF membrane receptors (normal human fibroblasts and human carcinomas).