CD147 and matrix metalloproteinase-2 protein expression as significant prognostic factors in esophageal squamous cell carcinoma
CD147 and matrix metalloproteinase-2 protein expression as significant prognostic factors in esophageal squamous cell carcinoma
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DOI：
10.1002/cncr.20593
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发表时间：
2004-11-01
期刊：
影响因子：
6.2
通讯作者：
Urashima, M
中科院分区：
文献类型：
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作者：
Ishibashi, Y;Matsumoto, T;Urashima, M
背景。作者研究了基质金属蛋白酶-2 （MMP-2）及其诱导剂CD147在食管癌病变及周围组织中的存在是否有助于预测患者预后。应用抗cd147和抗mmp -2抗体对101例食管鳞状细胞癌组织进行免疫组化分析。CD147在癌性和发育不良病变中表达，而在正常组织中不表达。相比之下，MMP-2主要在癌灶旁的正常间质组织中检测到，但在部分患者的癌灶中也检测到。病理结果表明，正常组织中MMP-2染色强度与肿瘤浸润深度和疾病分期呈正相关，而癌组织中MMP-2染色与血管和淋巴管侵袭以及癌细胞未成熟分化呈正相关。使用比例风险模型，包括癌灶内CD147染色模式和临床癌症分期的信息，提高了预测患者预后的准确性。这些结果表明，通过简单的免疫组织化学染色检测CD147和MMP-2的表达可以进一步提高对侵袭肿瘤细胞病理生理的认识，并结合癌症分期，可以提高研究人员预测食管鳞状细胞癌患者预后的能力。(C) 2004年美国癌症协会。
BACKGROUND. The authors investigated whether the presence of matrix metalloproteinase-2 (MMP-2) and its inducer, CD147, in cancerous esophageal lesions and surrounding tissue might help to predict patient prognosis.METHODS. Tissue samples from 101 patients with esophageal squamous cell carcinoma were stained with anti-CD147 and anti-MMP-2 antibodies for immunohistochemical analysis.RESULTS. CD147 was expressed in cancerous and dysplastic lesions, but not in normal tissue. In contrast, MMP-2 was detected mainly in normal interstitial tissue adjacent to cancerous lesions, but it was detected also in cancerous lesions in some patients. Pathologic findings demonstrated that the intensity of MMP-2 staining in normal tissue was associated positively with the depth of tumor infiltration and the stage of disease, whereas MMP-2 staining in cancerous tissue was associated positively with vascular and lymphatic vessel invasion as well as with immature differentiation of cancer cells. Using a proportional hazard model, including information on CD147 staining patterns within cancerous lesions along with clinical cancer staging, improved the accuracy of predicting patient prognosis.CONCLUSIONS. These results suggested that measurement of CD147 and MMP-2 expression with simple immunohistochemical staining may enhance further the understanding of the pathophysiology of invading tumor cells and, when used in combination with cancer staging, may increase the ability of investigators to predict prognosis in patients with esophageal squamous cell carcinoma. (C) 2004 American Cancer Society.