Immunization with cDNA of a novel P-III type metalloproteinase from the rattlesnake Crotalus durissus durissus elicits antibodies which neutralize 69% of the hemorrhage induced by the whole venom
Immunization with cDNA of a novel P-III type metalloproteinase from the rattlesnake Crotalus durissus durissus elicits antibodies which neutralize 69% of the hemorrhage induced by the whole venom
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DOI：
10.1016/j.toxicon.2008.05.023
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发表时间：
2008-08-01
期刊：
影响因子：
2.8
通讯作者：
Alape-Giron, Alberto
中科院分区：
文献类型：
--
作者：
Azofeifa-Cordero, Gabriela;Arce-Estradaa, Viviana;Alape-Giron, Alberto
锌依赖性金属蛋白酶在蝰蛇咬伤出血过程中起重要作用。本文报道了一种新的P-III型金属蛋白酶cDNA的克隆和序列分析。将重组质粒用于基因枪系统加速DNA包被微粒的DNA免疫小鼠。结果表明，金、钨微粒对小鼠的免疫效果无显著差异。用编码金属蛋白酶的DNA免疫小鼠的血清池中和了C. D. Durissus毒液用编码金属蛋白酶的DNA和编码鼠IL-2的质粒的共免疫增加了显示针对Cd的特异性抗体应答的小鼠的数目。Durissus毒液抗原产生的抗体的中和能力表明，DNA免疫钨微粒可用于抗蛇毒血清生产的战略。(C)2008爱思唯尔有限公司保留所有权利。
Zinc-dependent metalloproteinases play a key role in the hemorrhage induced by viperid bite envenoming. in this work we report the cloning and sequencing of the cDNA from a novel P-III type metalloprotemase from Crotalus durissus durissus venom glands. The recombinant plasmid was used for DNA immunization in mice using accelerate DNA-coated microparticles with the Gene Gun system. The results showed that there is no significant difference in the efficiency of the immunization in mice when gold or tungsten microparticles were used. A pool of the sera from mice immunized with the metalloprotemase encoding DNA neutralized the hemorrhagic activity of C. d. durissus venom. The coimmunization with DNA encoding the metalloproteinase and a plasmid encoding the murine IL-2 increased the number of mice which show a specific antibody response towards C d. durissus venom antigens. The neutralizing ability of the produced antibodies demonstrates that DNA immunization with tungsten microparticles may be used in strategies for antivenom production. (C) 2008 Elsevier Ltd. All rights reserved.