Reversible inhibition of ethylene action and interruption of petal senescence in carnation flowers by norbornadiene.
Reversible inhibition of ethylene action and interruption of petal senescence in carnation flowers by norbornadiene.
复制标题
降冰片二烯对康乃馨花乙烯作用的可逆抑制和花瓣衰老的中断。
作者：
Hong Wang;W. Woodson
以香石竹（DianthuscaryophyllusL.）为试材，研究了环烯烃2，5-降冰片二烯（NBD）对乙烯作用的抑制效应。cv白色Sim）花。在每升500微升NBD的存在下，在开花期用乙烯处理花朵，增加了引发反应（花瓣衰老）所需的乙烯浓度，这表明NBD表现为乙烯作用的竞争性抑制剂。将产生自催化乙烯并表现出衰老迹象（花瓣内卷）的花转移到NBD的气氛中，导致乙烯产生、花瓣1-氨基环丙烷-1-羧酸合酶活性、1-氨基环丙烷-1-羧酸含量和乙烯形成酶活性迅速降低。NBD的去除导致乙烯生物合成的恢复。这些结果支持花瓣衰老的跃变型阶段的乙烯产生的自催化调节，并表明乙烯的持续感知是维持乙烯生物合成所必需的。NBD抑制乙烯作用后，花已达到更年期高峰与花瓣衰老的逆转衰老症状所证明的中断。这一结果与人们普遍认为的一旦花瓣进入乙烯跃变期，花瓣衰老的速率是固定的和不可逆的相反。
The inhibitory effects of the cyclic olefin 2,5-norbornadiene (NBD) on ethylene action were tested in carnation (Dianthus caryophyllus L. cv White Sim) flowers. Treatment of flowers at anthesis with ethylene in the presence of 500 microliters per liter NBD increased the concentration of ethylene required to elicit a response (petal senescence), indicating that NBD behaves as a competitive inhibitor of ethylene action. Transfer of flowers producing autocatalytic ethylene and exhibiting evidence of senescence (petal in-rolling) to an atmosphere of NBD resulted in a rapid reduction in ethylene production, petal 1-aminocyclopropane-1-carboxylic acid synthase activity, 1-aminocyclopropane-1-carboxylic acid content, and ethylene forming enzyme activity. Removal of NBD resulted in recovery of ethylene biosynthesis. These results support the autocatalytic regulation of ethylene production during the climacteric stage of petal senescence and suggest that continued perception of ethylene is required for maintenance of ethylene biosynthesis. The inhibition of ethylene action by NBD after the flowers had reached the climacteric peak was associated with interruption of petal senescence as evidenced by reversal of senescence symptoms. This result is in contrast to the widely held belief that the rate of petal senescence is fixed and irreversible once petals enter into the ethylene climacteric.