High-resolution mapping of quantitative trait loci in outbred mice
High-resolution mapping of quantitative trait loci in outbred mice
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DOI：
10.1038/6825
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发表时间：
1999-03-01
期刊：
影响因子：
30.8
通讯作者：
Flint, J
中科院分区：
文献类型：
--
作者：
Talbot, CJ;Nicod, A;Flint, J
Screening the whole genome of a cross between two inbred animal strains has proved to be a powerful method for detecting genetic loci underlying quantitative behavioural traits(1-7), but the level of resolution offered by quantitative trait loci (QTL) mapping is still too coarse to permit molecular cloning of the genetic determinants(8). To achieve high-resolution mapping, we used an outbred stock of mice for which the entire genealogy is known. The heterogeneous stock (HS) was established 30 years ago from an eight-way cross of C57BL/6, BALB/c, RIII, AKR, DBA/2, I, All and C3H inbred mouse strains(9). At the time of the experiment reported here, the HS mice were at generation 58, theoretically offering at least a 30-fold increase in resolution for QTL mapping compared with a backcross or an F2 intercross(10,11). Using the HS mice we have mapped a QTL influencing a psychological trait in mice to a 0.8-cM interval on chromosome 1. This method allows simultaneous fine mapping of multiple QTLs, as shown by our report of a second QTL on chromosome 12. The high resolution possible with this approach makes QTLs accessible to positional cloning.
