GABAergic parvalbumin-immunoreactive large calyciform presynaptic complexes in the reticular nucleus of the rat thalamus
GABAergic parvalbumin-immunoreactive large calyciform presynaptic complexes in the reticular nucleus of the rat thalamus
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DOI：
10.1016/j.jchemneu.2005.03.010
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发表时间：
2005-07-01
影响因子：
2.8
通讯作者：
Fenyo, R
中科院分区：
文献类型：
--
作者：
Csillik, B;Mihály, A;Fenyo, R
在大鼠丘脑网状核内，几乎所有神经元都呈小白蛋白免疫反应。此外，我们还发现，虽然表面上类似于大的小白蛋白免疫反应神经元，但如核变化曲线所示，在丘脑网状核中也存在许多特殊的小白蛋白免疫反应复合体，这些复合体与小白蛋白免疫反应周围区不同。光镜和电子显微镜免疫细胞化学研究表明，这些小白蛋白免疫反应复合体是由小白蛋白免疫反应的帽状终末形成的，这些终末与小白蛋白免疫阴性的大树突形成突触。切断丘脑-网状连接不会引起丘脑网状核内帽状终末的改变。核反染色显示，小白蛋白免疫反应的帽状终末起源于局部的小白蛋白免疫反应神经元间质，它们根据从核周突出的“颈”的长度，建立体树突触、轴树突触或树突突触。光镜和电子显微镜免疫细胞化学研究证明，小白蛋白免疫反应的帽状复合体也含有可能具有抑制作用的GABA。根据文献资料，我们的结果表明，丘脑网状核内具有小白蛋白免疫反应的GABA能帽状终末可能参与细胞与细胞之间的内在联系，因此可能参与丘脑-皮质振荡的同步化、睡眠纺锤波的产生和注意过程。(C)2005 Elsevier B.V.保留所有权利。
In the reticular thalamic nucleus of the rat, nearly all neurons are parvalbumin-immunoreactive. We found that in addition, though superficially similar to large parvalbumin-immunoreactive neurons, also numerous peculiar parvalbumin-immunoreactive complexes are present in the reticular thalamic nucleus which are not identical with parvalbumin-immunoreactive perikarya, as shown by nuclear variation curves. Light and electron microscopic immunocytochemical studies revealed that these parvalbumin-immunoreactive complexes are brought about by parvalbumin-immunoreactive calyciform terminals which establish synapses with large, parvalbumin-immunonegative dendritic profiles. Transection of thalamo-reticular connections did not cause any alteration of calyciform terminals in the reticular thalamic nucleus. Nuclear counterstaining revealed that parvalbumin-immunoreactive calyciform terminals originated from local parvalbumin-immunoreactive interneuronal perikarya, which, depending of the length of the "neck" protruding from the perikaryon, establish somato-dendritic, axo-dendritic or dendro-dendritic synapses. Light and electron microscopic immunocytochemical investigations prove that the parvalbumin-immunoreactive calyciform complexes contain also GABA, that are likely to be inhibitory. In accordance with literature data, our results suggest that parvalbumin-immunoreactive GABAergic calyciform terminals in the reticular thalamic nucleus may be instrumental in intrinsic cell-to-cell communications and, as such, may be involved in synchronisation of thalamo-cortical oscillations, in the production of sleep spindles and in attentional processes. (c) 2005 Elsevier B.V. All rights reserved.