Growth-inhibiting antibody responses of humans vaccinated with recombinant outer surface protein A or infected with Borrelia burgdorferi or both.
Growth-inhibiting antibody responses of humans vaccinated with recombinant outer surface protein A or infected with Borrelia burgdorferi or both.
复制标题
接种重组外表面蛋白 A 或感染伯氏疏螺旋体或两者的人类的生长抑制抗体反应。
DOI：
10.1086/315359
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Barbour,AG
中科院分区：
文献类型：
--
作者：
Luke,CJ;Marshall,MA;Zahradnik,JM;Bybel,M;Menefee,BE;Barbour,AG
用伯氏疏螺旋体生长抑制试验(GIA)和抗OspA酶联免疫吸附试验(EL ISA)分析了外膜蛋白(OSP)A疫苗两年人体试验的系列血清样本，以评估疫苗接受者和莱姆病患者的抗体反应。虽然74%的OspA接受者在接种3次疫苗后GIA滴度与相当，但安慰剂接受者中没有一人有如此高的GIA滴度，即使是那些患有莱姆病的人。接种3次疫苗后，GIA和EL ISA滴度之间的相关性为.84；然而，更多的疫苗接受者的EL ISA滴度高且GIA滴度低，而EL ISA滴度低且GIA滴度高。获得莱姆病的OspA疫苗接受者在3次免疫后血清GIA和ELISA效价显著低于年龄和性别匹配的无莱姆病的OspA接受者。因此，接种疫苗的受试者在活细胞上有针对天然抗原的抗体，具有这种特异性的抗体检测可以预测疫苗接种者对感染的保护。
Serial serum samples from a 2-year human trial of outer surface protein (Osp) A vaccine were analyzed byBorrelia burgdorferigrowth-inhibition assay (GIA) and anti-OspA ELISA to assess the antibody responses of vaccine recipients and subjects with Lyme disease. Although 74% of OspA recipients had a reciprocal GIA titer ⩾64 after 3 vaccinations, none of the placebo recipients, even those with Lyme disease, had a GIA titer this high. The correlation between GIA and ELISA titers after 3 doses of vaccine was .84; however, more vaccine recipients had an elevated ELISA titer paired with low GIA titer than had a low ELISA titer with a high GIA titer. OspA-vaccine recipients who acquired Lyme disease had significantly lower serum GIA and ELISA titers after 3 immunizations than did age- and sex-matched OspA recipients without Lyme disease. Thus, vaccinated subjects had antibodies to native antigen on viable cells, and antibody assays with this specificity may predict protection of vaccinees against infection.