OSMOTICALLY INDUCED ELECTRICAL SIGNALS FROM ACTIN-FILAMENTS
OSMOTICALLY INDUCED ELECTRICAL SIGNALS FROM ACTIN-FILAMENTS
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DOI：
10.1016/s0006-3495(91)82343-8
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发表时间：
1991-06-01
影响因子：
3.4
通讯作者：
ZANER, K
中科院分区：
文献类型：
--
作者：
CANTIELLO, HF;PATENAUDE, C;ZANER, K
肌动蛋白丝（F-actin）是皮质细胞骨架的主要组成部分，在多种细胞功能中发挥着重要作用。在本报告中，我们评估了渗透应激对 F-肌动蛋白电化学特性的作用。聚合肌动蛋白溶液 (5 mg/ml) 的自发唐南电位为 -3.93 +/- 1.84 mV，渗透压会线性降低 1-20 mOsm (0.28 +/- 0.06 mV/mM)。计算出的表面电荷密度会降低，并最终通过增加渗透应力来逆转，正如相变行为所预期的那样。 F-肌动蛋白的电渗行为在 pH 5.5 时消失，并且取决于其丝状性质。此外，渗透压应激的 F-肌动蛋白在施加 500-2,000 V/cm 量级的电场时表现出非线性电响应。这些数据表明溶液中的F-肌动蛋白可能表现出与离子“电缆”行为一致的非理想电渗特性，这在细胞室内电信号的处理和传导中可能具有生物学意义。
Actin filaments, F-actin, a major component of the cortical cytoskeleton, play an important role in a variety of cell functions. In this report we have assessed the role of osmotic stress on the electrochemical properties of F-actin. The spontaneous Donnan potential of a polymerized actin solution (5 mg/ml) was -3.93 +/- 1.84 mV, which was linearly reduced by osmotic stress on the order of 1-20 mOsm (0.28 +/- 0.06 mV/mM). Calculated surface charge density was reduced and eventually reversed by increasing the osmotic stress as expected for a phase transition behavior. The electro-osmotic behavior of F-actin disappeared at pH 5.5 and was dependent on its filamentous nature. Furthermore, osmotically stressed F-actin displayed a nonlinear electric response upon application of electric fields on the order of 500-2,000 V/cm. These data indicate that F-actin in solution may display nonideal electro-osmotic properties consistent with ionic "cable" behavior which may be of biological significance in the processing and conduction of electrical signals within the cellular compartment.