The adenovirus protein Gam1 interferes with sumoylation of histone deacetylase 1
The adenovirus protein Gam1 interferes with sumoylation of histone deacetylase 1
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DOI：
10.1093/embo-reports/kvf213
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发表时间：
2002-11-01
期刊：
影响因子：
7.7
通讯作者：
Chiocca, S
中科院分区：
文献类型：
--
作者：
Colombo, R;Boggio, R;Chiocca, S
腺病毒早期基因产物Gam 1对病毒复制至关重要，并通过灭活组蛋白去乙酰化酶1（HDAC 1）诱导某些细胞基因。我们证明，Gam 1（i）破坏前髓细胞白血病核小体，（ii）将SUMO-1离域到细胞质中，（iii）影响SUMO-1通路。此外，我们表明，Gam 1抵消HDAC 1 SUMO化在体内和体外。HDAC 1的SUMO化似乎不是HDAC 1生物活性所绝对需要的，而是复杂调节回路的一部分，该回路还包括脱乙酰基酶的磷酸化。我们的数据表明，Gam 1是一种病毒蛋白，可以同时影响两个信号通路：类小泛素化和乙酰化。
The adenovirus early gene product Gam1 is crucial for virus replication and induces certain cellular genes by inactivating histone deacetylase 1 (HDAC1). We demonstrate that Gam1 (i) destroys promyelocitic leukemia nuclear bodies, (ii) delocalizes SUMO-1 into the cytoplasm and (iii) influences the SUMO-1 pathway. In addition, we show that Gam1 counteracts HDAC1 sumoylation both in vivo and in vitro. Sumoylation of HDAC1 does not seem to be absolutely required for HDAC1 biological activity but is part of a complex regulatory circuit that also includes phosphorylation of the deacetylase. Our data demonstrate that Gam1 is a viral protein that can affect simultaneously two signaling pathways: sumoylation and acetylation.