Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica.
Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica.
复制标题
从南极洲斯卡维斯内斯无冰区的土壤和湖泊沉积物中获得的真菌的冷适应能力。
DOI：
10.1111/1574-6968.12217
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发表时间：
2013
影响因子：
2.1
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Tsuji;M.;Fujiu;S.;Xiao N;Hanada;Y.;Kudoh;S.;Kondo;H.;Tsuda;S.,and Hoshino;T.
从南极洲斯卡夫斯内斯地区湖泊沉积物和湖泊周围土壤中采集到71个分离物。根据ITS区序列的相似性将这些分离物分为10个属。23个菌株被归类为子囊菌5属（Embellisia，Phoma，Geomyces，TetracladiumorThelebolus）和48个菌株被归类为担子菌5属（Mrakia，Cryptococcus，Dioszegia，Rhodotorulum Leucosporidium）。35%的可培养真菌属于Mrakia属。从8个属中选择了18个菌株，并测试了抗冻活性和在− 1至25 ° C温度范围内的生长能力。NHT-2与R. gracialis，Leucosporidiumsp. BSS-1与Leu具有高度的同源性。Anacriticum（Glaciozyma anacritica），并且这两个分离物表现出抗冻活性。检测的所有分离株都能在-1 °C下生长。虽然能够在-1 °C下生长，但没有表现出任何抗冻活性，并且仅表现出有限的胞外多糖分泌。Mrakia属的物种具有大量的不饱和脂肪酸，表明该属的成员通过增加膜流动性来适应寒冷环境。
A total of 71 isolates were collected from lake sediment and soil surrounding lakes in the Skarvsnes area, Antarctica. Based on ITS region sequence similarity, these isolates were classified to 10 genera. Twenty-three isolates were categorized as ascomycetous fungi from five genera (Embellisia, Phoma, Geomyces, TetracladiumorThelebolus) and 48 isolates were categorized as basidiomycetous fungi in five genera (Mrakia, Cryptococcus, Dioszegia, RhodotorulaorLeucosporidium). Thirty-five percent of culturable fungi were of the genusMrakia. Eighteen isolates from eight genera were selected and tested for both antifreeze activity and capacity for growth under temperatures ranging from −1 to 25 °C.Rhodotorulasp. NHT-2 possessed a high degree of sequence homology withR. gracialis, whileLeucosporidiumsp. BSS-1 possessed a high degree of sequence homology withLeu. antarcticum(Glaciozyma antarctica), and these two isolates demonstrated antifreeze activity. All isolates examined were capable of growth at −1 °C.Mrakiaspp., while capable of growth at −1 °C, did not demonstrate any antifreeze activity and exhibited only limited secretion of extracellular polysaccharides. Species of the genusMrakiapossessed high amounts of unsaturated fatty acids, suggesting that members of this genus have adapted to cold environments by increasing their membrane fluidity.