Circular dichroism of liver alcohol dehydrogenase complexes with auramine O.

Circular dichroism of liver alcohol dehydrogenase complexes with auramine O.
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肝醇脱氢酶与金胺 O 复合物的圆二色性。

DOI:
10.1021/bi00742a016
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发表时间:
1973
期刊:
影响因子:
2.9
通讯作者:
R. Woody
R. Woody
中科院分区:
生物学3区
文献类型:
--
作者:
J. S. Wicken;R. Woody

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Materials and MethodsReagents. Crystalline liver alcohol dehydrogenase was pur-chased from Sigma Chemical Co., St. Louis, Mo., and dialyzed against 0.1 m sodium phosphate buffer at pH 7.4 as described by Brand et al.(1967). Enzyme concentration was determined from the absorbance at 280 nm, using a molar extinction co-biochemistry, vol. 12, no. 1 8, 1973 3459