Human metapneumovirus in infants and young children in Thailand with lower respiratory tract infections; molecular characteristics and clinical presentations
Human metapneumovirus in infants and young children in Thailand with lower respiratory tract infections; molecular characteristics and clinical presentations
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DOI：
10.1016/j.jinf.2005.07.001
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发表时间：
2006-04-01
影响因子：
28.2
通讯作者：
Poovorawan, Y
中科院分区：
文献类型：
--
作者：
Samransamruajkit, R;Thanasugarn, W;Poovorawan, Y
人偏肺病毒（Human metapneumovirus，hMPV）是近年来发现的一种副粘病毒。亚洲人群中hMPV的临床特征和分子特征至今仍不清楚。目的：本研究旨在调查婴儿和幼儿急性下呼吸道感染（ALRI）中hMPV的患病率，并确定其分子特征和临床表现。从2001年3月至2003年9月，在朱拉隆功国王纪念医院收集了236例患有ALRI的婴儿和儿童的鼻咽分泌物（NP）。应用特异性引物进行逆转录-聚合酶链反应（RT-PCR）鉴定hMPV和hRSV。结果：236例婴幼儿中hMPV N、F、L基因阳性12例，阳性率为5.4%。hMPV感染儿童的平均年龄为22 ± 11个月。主要临床表现为发热伴咳嗽（100%）和上呼吸道症状（10/12，83%）。12名婴儿中有11名（92%）住院治疗。此外，系统发育分析确定了两个不同的谱系hMPV。结论：我们的研究结果表明，在泰国的婴儿和幼儿下呼吸道感染的hMPV感染的患病率，分子特征和临床谱。(C)2005年，英国传染病协会。由爱思唯尔有限公司发布。保留所有权利。
Human metapneumovirus (hMPV) is a recently identified Paramyxovirus. The clinical features and molecular characteristics of hMPV in Asian populations have so far remained obscure.Objective: The present study was designed to investigate the prevalence of hMPV in infants and young children presented with acute lower respiratory tract infection (ALRI) and to identify the molecular characteristics and clinical presentations.Methods: There were 236 nasopharyngeal secretions (NPs) collected from infants and children presented with ALRI at King Chulatongkorn Memorial hospital between March 2001 and September 2003. Reverse transcriptase-polymerase chain reaction (RT-PCR) applying specific primers was done to identify hMPV and hRSV. Phylogenetic analysis of hMPV N, F and L genes was also performed.Results: Of the 220 (236) infants and young children tested, positive results were found in 12 specimens (5.4%). The mean age of children with hMPV infections was 22 +/- 11 months. They mostly presented with fever with cough (100%) and upper respiratory tract symptoms (10/12, 83%). Eleven of twelve infants (92%) were hospitalized. Additionally, phylogenetic analysis identified two distinct lineages of hMPV.Conclusion: Our results demonstrated the prevalence, molecular characterization and clinical spectrum of hMPV infection in infants and young children presented with lower respiratory tract infections in Thailand. (C) 2005 The British Infection Society. Published by Elsevier Ltd. All rights reserved.