Procyanidin dimer B2 [epicatechin-(4β-8)-epicatechin] in human plasma after the consumption of a flavanol-rich cocoa
Procyanidin dimer B2 [epicatechin-(4β-8)-epicatechin] in human plasma after the consumption of a flavanol-rich cocoa
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DOI：
10.1093/ajcn/76.4.798
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发表时间：
2002-10-01
影响因子：
7.1
通讯作者：
Keen, CL
中科院分区：
文献类型：
--
作者：
Holt, RR;Lazarus, SA;Keen, CL
背景：流行病学研究将富含类黄酮的食物与心血管死亡率的降低联系起来。一些可可粉是富含类黄酮的可可，并包含由这些单体单位形成的单体黄酮（ - ） - epicatechin和（+） - 儿茶素和寡聚的procyanidins。单体和低聚物在体外和初步临床研究中都表现出有利影响心血管健康的潜力。尽管先前的研究表明，可可消耗后人类血浆中的（ - ） - epicatechin的浓度增加，但在已发表的文献中没有关于人血浆中procyanidins存在的信息。对象：本研究试图确定是否可以检测到procyanidins急性消耗富含黄麻醇的可可症后，在人血浆中进行了量化。设计：外周血是从5个健康的成人受试者（基线，0 h）和0.5、2和6小时的0.375 g可可/千克体内WT后获得的。作为饮料。分析血浆样品中的单体和procyanidins，使用使用库米电化学阵列检测的反相HPLC和液相色谱 - 串联质谱法。回测：procyanidin dimer，（ - ） - （ - ） - Epicatechin和（+） - 尾techin和（+） - 省尾触及尾catechin dimer。急性可可消耗后，人类受试者的血浆早在0.5 h（16 5 nmol/l，2.61 +/- 0.46 mumol/l，分别分别为0.13 +/- 0.03 mumol/l），并达到最大浓度2 h（41 h（41） +/- 4 nmol/l，5.92 +/- 0.60 mumol/l，分别为0.16 +/- 0.03 mumol/l）。结论：二聚体procyanidins在消耗后30分钟内可以在人血浆中检测到二聚体procyanidins富含黄酮醇的食物，例如可可。
Background: Epidemiologic studies have linked flavonoid-rich foods with a reduced risk of cardiovascular mortality. Some cocoas are flavonoid-rich and contain the monomeric flavanols (-)-epicatechin and (+)-catechin and oligomeric procyanidins formed from these monomeric units. Both the monomers and the oligomers have shown potential in favorably influencing cardiovascular health in in vitro and preliminary clinical studies. Although previous investigations have shown increasing concentrations of (-)-epicatechin in human plasma after cocoa consumption, no information is available in the published literature regarding the presence of procyanidins in human plasma.Objective: This study sought to determine whether procyanidins can be detected and quantified in human plasma after acute consumption of a flavanol-rich cocoa.Design: Peripheral blood was obtained from 5 healthy adult subjects before (baseline, 0 h) and 0.5, 2, and 6 h after consumption of 0.375 g cocoa/kg body wt as a beverage. Plasma samples were analyzed for monomers and procyanidins with the use of reversed-phase HPLC with coulometric electrochemical array detection and liquid chromatography-tandem mass spectrometry.Results: Procyanidin dimer, (-)-epicatechin, and (+)-catechin were detected in the plasma of human subjects as early as 0.5 h (16 5 nmol/L, 2.61 +/- 0.46 mumol/L, and 0.13 +/- 0.03 mumol/L, respectively) after acute cocoa consumption and reached maximal concentrations by 2 h (41 +/- 4 nmol/L, 5.92 +/- 0.60 mumol/L, and 0.16 +/- 0.03 mumol/L, respectively).Conclusion: Dimeric procyanidins can be detected in human plasma as early as 30 min after the consumption of a flavanol-rich food such as cocoa.