An LRR Receptor-Teneurin System Directs Planar Polarity at Compartment Boundaries.
An LRR Receptor-Teneurin System Directs Planar Polarity at Compartment Boundaries.
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LRR 受体-Teneurin 系统在区室边界处引导平面极性。
DOI：
10.1016/j.devcel.2019.08.003
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发表时间：
2019
影响因子：
11.8
通讯作者：
Zallen,JenniferA
中科院分区：
文献类型：
--
作者：
Paré,AdamC;Naik,Pooja;Shi,Jay;Mirman,Zachary;Palmquist,KarlH;Zallen,JenniferA
上皮细胞响应细胞表面受体传递的细胞外空间线索而动态地自组织。在果蝇的会聚伸展过程中，Toll相关受体引导平面极化的细胞重排，拉长了头尾轴线。然而，许多细胞在没有Toll受体活性的情况下建立了极性，这表明存在额外的空间线索。在这里，我们证明了富含亮氨酸的重复受体Tartan和Teneurin Ten-m在上皮室边界提供了关键的极性信号。Tartan和Ten-m胞外区在体外相互作用，Tartan在体内促进Ten-m定位到房室边界。我们证明了Tartan和Ten-m对于隔室边界单元的平面极性和组织是必要的。此外，格纹和Ten-m的异位条纹足以诱导肌球蛋白在条纹边界聚集。这些结果表明，Tartan/Ten-m和Toll受体系统共同创建了一个高分辨率的空间线索网络，指导细胞在会聚延伸过程中的行为。
Epithelial cells dynamically self-organize in response to extracellular spatial cues relayed by cell-surface receptors. During convergent extension inDrosophila, Toll-related receptors direct planar polarized cell rearrangements that elongate the head-to-tail axis. However, many cells establish polarity in the absence of Toll receptor activity, indicating the presence of additional spatial cues. Here we demonstrate that the leucine-rich-repeat receptor Tartan and the teneurin Ten-m provide critical polarity signals at epithelial compartment boundaries. The Tartan and Ten-m extracellular domains interactin vitro, and Tartan promotes Ten-m localization to compartment boundariesin vivo. We show that Tartan and Ten-m are necessary for the planar polarity and organization of compartment boundary cells. Moreover, ectopic stripes of Tartan and Ten-m are sufficient to induce myosin accumulation at stripe boundaries. These results demonstrate that the Tartan/Ten-m and Toll receptor systems together create a high-resolution network of spatial cues that guides cell behavior during convergent extension.