DIFFERENTIAL RESPONSE OF HUMAN AND RODENT CELL-LINES TO CHEMICAL INHIBITION OF THE REPAIR OF POTENTIALLY LETHAL DAMAGE
DIFFERENTIAL RESPONSE OF HUMAN AND RODENT CELL-LINES TO CHEMICAL INHIBITION OF THE REPAIR OF POTENTIALLY LETHAL DAMAGE
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DOI：
10.1007/bf01211256
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发表时间：
1989-01-01
影响因子：
1.7
通讯作者：
DAHLBERG, WK
中科院分区：
文献类型：
--
作者：
LITTLE, JB;UENO, AM;DAHLBERG, WK
我们研究了几类代谢抑制剂对两种啮齿动物和两种人类细胞系统（其生长特性不同）的密度抑制培养物中潜在致命损伤修复的影响。阿菲迪霉素、1-β-D-阿拉伯呋喃糖基胞嘧啶(ara-C)和羟基脲对PLD修复没有作用，而9-β-D-阿拉伯呋喃糖腺嘌呤(ara-A)和3-氨基苯甲酰胺(3-AB)的作用是细胞系依赖性的。例如，3-AB 在 CHO 细胞中几乎完全抑制 PLD 修复，但在人二倍体成纤维细胞中没有显示出抑制作用。这些结果表明DNA复制和聚(ADP-核糖)合成的抑制剂并不是受辐射细胞中细胞恢复的有效抑制剂，而且这种作用可能是细胞系依赖性的。
We have examined the effects of several classes of metabolic inhibitors on the repair of potentially lethal damage in density-inhibited cultures of two rodent and two human cell systems which differ in their growth characteristics. Aphidicolin, 1-.beta.-D-arabinofuranosylcytosine (ara-C) and hydroxyurea showed no effect on PLD repair, whereas the effects of 9-.beta.-D-arabinofuranosyladenine (ara-A) and 3-aminobenzamide (3-AB) were cell line dependent. For example, 3-AB suppressed PLD repair almost completely in CHO cells, but showed no inhibitory effects in human diploid fibroblasts. These results indicate that inhibitors of DNA replication and poly(ADP-ribose) synthesis are not efficient inhibitors of cellular recovery in irradiated cells and, moreover, that such effects may be cell line dependent.