COX-2 mRNA Expression is Significantly Increased in Acid-exposed Compared to Nonexposed Squamous Epithelium in Gastroesophageal Reflux Disease
COX-2 mRNA Expression is Significantly Increased in Acid-exposed Compared to Nonexposed Squamous Epithelium in Gastroesophageal Reflux Disease
复制标题
胃食管反流病中，与未暴露的鳞状上皮相比，暴露于酸的鳞状上皮中 COX-2 mRNA 表达显着增加
DOI：
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发表时间：
2007
影响因子：
3.2
通讯作者：
P. Schneider
中科院分区：
文献类型：
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作者：
G. Lurje;D. Vallbohmer;P. Collet;H. Xi;S. Baldus;J. Brabender;R. Metzger;Michaela Heitmann;S. Neiss;Ute Drebber;A. Holscher;P. Schneider
背景环氧合酶(COX)-2在胃食道反流病(GERD)和Barrett化生中的作用尚不清楚。本研究的目的是进一步分析COX-2mRNA在胃肠道反流病(GERD)患者中的表达，并与Barrett‘s食道(BE)和Barrett癌(BC)进行比较。方法在常规上消化道内窥镜检查中获取胃肠道反流病(n = 43例)、BE(n = 20例)和BC(n = 47例)患者的组织标本。实时定量逆转录聚合酶链式反应(RT-PCR)检测COX-2的表达水平。结果COX-2mRNA在胃肠上皮化生-异型增生-腺癌序列中呈进行性上调(p = 0.001)。DeMeester评分为14.72分的GERD患者远端鳞状上皮中COX-2的表达水平显著高于DeMeester评分正常的患者(p = 0.01)。结论COX-2mRNA表达的改变与胃反流病的胃镜或组织学征象无关。然而，由于未知原因，这种早期COX-2在GERD中的增加在MDA序列中进一步上调。
BackgroundLittle is known about the role of cyclooxygenase (COX)-2 in gastroesophageal reflux disease (GERD) and the development of Barrett’s metaplasia. The objectives of this study were to further analyze COX-2 mRNA expression in patients with GERD compared to Barrett’s esophagus (BE) and Barrett’s cancer (BC).MethodsTissue samples from 110 patients with GERD (n = 43), BE (n = 20), and BC (n = 47) were obtained in routine upper GI endoscopy. Expression levels of COX-2 were measured by quantitative real-time reverse trancriptase polymerase chain reaction (RT-PCR). Also, 24-h pH monitoring was performed in all patients of the GERD study group and the DeMeester composite score was used to match COX-2 mRNA expression with the severity of acid exposure in the lower esophagus.ResultsCOX-2 mRNA is progressively upregulated within the metaplasia–dysplasia–adenocarcinoma (MDA) sequence (p = 0.001). COX-2 levels of the squamous epithelium in the distal esophagus from patients with GERD and a pathologic mean DeMeester score (>14.72) were significantly higher than in patients with normal DeMeester scores (p = 0.01).ConclusionIn summary our findings suggest that alterations in COX-2 mRNA expression occur independently of endoscopic or histologic signs of GERD in the acid-exposed squamous epithelium of the distal esophagus. However, this early COX-2 increase in GERD is further upregulated within the MDA sequence for yet unknown reasons.
影响因子：
29.4
作者：
Buttar, NS;Wang, KK;Burgart, LJ
通讯作者：
Burgart, LJ
影响因子：
158.5
作者：
Lagergren, J;Bergström, R;Nyrén, O
通讯作者：
Nyrén, O