Potent antibacterial activity of halogenated metabolites from Malaysian red algae, Laurencia majuscula (Rhodomelaceae, Ceramiales)
Potent antibacterial activity of halogenated metabolites from Malaysian red algae, Laurencia majuscula (Rhodomelaceae, Ceramiales)
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DOI：
10.1016/s1389-0344(03)00067-4
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发表时间：
2003-07-01
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影响因子：
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通讯作者：
Vairappan, CS
中科院分区：
文献类型：
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作者：
Vairappan, CS
凹顶藻属（Laurencia）是红藻目（Rhodomelaceae，Ceramiales）的一种重要的次生卤代代谢产物。本研究探讨了从马来西亚沙巴沃茨两个地点采集的大凹顶藻（Laurencia majuscula Harvey）内在次生代谢产物的提取、分离、结构鉴定和抗菌活性。分离出两个主要的卤代化合物，分别鉴定为elatol（1）和iso-obtusol（2）。通过IR、~ 1H-NMR、~（13）C-NMR和旋光度等波谱数据确定了化合物的结构。采用纸片扩散法（Kirby-Bauer法）进行对人体致病菌的抗菌生物测定。艾拉酚（1）对6种细菌有抑制作用，对表皮葡萄球菌有明显的抑菌作用，结果表明，异obtusol（2）对肺炎克雷伯氏菌和沙门氏菌有较强的抗菌活性，其中对肺炎克雷伯氏菌和沙门氏菌有较强的抗菌活性。仅相当于商业抗生素对肺炎克雷伯氏菌和沙门氏菌的效力。使用稀释法进行的进一步测试显示，两种化合物对测试细菌具有抑菌作用模式。(C)2003 Elsevier Science B. V.保留所有权利。
Red algae genus Laurencia (Rhodomelaceae, Ceramiales) are known to produce a wide range of chemically interesting secondary halogenated metabolites. This investigation delves upon extraction, isolation, structural elucidation and antibacterial activity of inherently available secondary metabolites of Laurencia majuscula Harvey collected from two locations in waters of Sabah, Malaysia. Two major halogenated compounds, identified as elatol (1) and iso-obtusol (2) were isolated. Structures of these compounds were determined from their spectroscopic data such as IR, H-1-NMR, C-13-NMR and optical rotation. Antibacterial bioassay against human pathogenic bacteria was conducted using disc diffusion (Kirby-Bauer) method. Elatol (1) inhibited six species of bacteria, with significant antibacterial activities against Staphylococcus epidermis, Klebsiella pneumonia and Salmonella sp. while iso-obtusol (2) exhibited antibacterial activity against four bacterial species with significant activity against K pneumonia and Salmonella sp. Elatol (1) showed equal and better antibacterial activity compared with tested commercial antibiotics while iso-obtusol (2) only equaled the potency of commercial antibiotics against K pneumonia and Salmonella sp. Further tests conducted using dilution method showed both compounds as having bacteriostatic mode of action against the tested bacteria. (C) 2003 Elsevier Science B.V. All rights reserved.