The role of the OsCam1-1 salt stress sensor in ABA accumulation and salt tolerance in rice
The role of the OsCam1-1 salt stress sensor in ABA accumulation and salt tolerance in rice
复制标题
DOI：
10.1007/s12374-011-0154-8
复制
发表时间：
2012-06-01
影响因子：
2.9
通讯作者：
Chadchawan, Supachitra
中科院分区：
文献类型：
--
作者：
Saeng-ngam, Sukhumaporn;Takpirom, Warintra;Chadchawan, Supachitra
研究了盐诱导钙调素基因OsCam 1 -1对盐胁迫下水稻品种“Khoa Dawk Mali 105”（KDML 105）脱落酸（阿坝）生物合成的影响。FL 530-IL是从KDML 105衍生的等基因耐盐系，盐胁迫处理后叶片中阿坝的积累量是KDML 105的2.9倍。盐胁迫30 min后，FL 530-IL和KDML 105水稻品种叶片中OsCam 1 -1的转录水平分别是非盐胁迫植株的24倍和7倍。与野生型和不表达OsCam 1 - 1的对照转基因株系相比，组成型过表达OsCam 1 -1的转基因株系能够上调阿坝醛氧化酶和9-顺式-环氧类胡萝卜素双加氧酶3这两个参与阿坝生物合成的基因，并具有更高的阿坝含量。此外，过量表达OsCam 1 -1的转基因植物对盐胁迫的耐受性更强，例如，在盐胁迫期间保持其地上部和根质量（以干重计）的能力比对照植物更好。这些数据表明OsCam 1 -1信号转导可能在阿坝生物合成中起重要作用，OsCam 1 -1基因的表达水平和阿坝积累可能与水稻的耐盐性有关。
Involvement of the salt-inducible calmodulin gene, OsCam1-1, in abscisic acid (ABA) biosynthesis during salt stress was studied in the 'Khoa Dawk Mali 105' (KDML105) rice cultivar (Oryza sativa L.). FL530-IL, an isogenic salt-resistant line derived from the KDML105 cultivar, accumulated a 2.9-fold higher concentration of ABA in the leaves after salt stress treatment than that for KDML105. A twenty-four and a seven- fold higher level of OsCam1-1 transcripts were detected in the leaves of the FL530-IL and KDML105 rice cultivars, respectively, after 30 min of salt stress compared to non-salt-stressed plants. Transgenic rice lines that constitutively over-express the OsCam1-1 gene were found to up-regulate ABA aldehyde oxidase and 9-cis-epoxycarotenoid dioxygenase 3, two genes involved in ABA biosynthesis, and to have a higher ABA content, when compared to the wild type and the control transgenic lines without OsCam1-1 over-expression. In addition, transgenic plants over-expressing OsCam1-1 were more tolerant to salt stress, with, for example, a better ability to maintain their shoot and root mass (as dry weight) during salt stress, than the control plants. These data indicate that OsCam1-1 signaling is likely to play an important role in ABA biosynthesis, and the level of OsCam1-1 gene expression and ABA accumulation probably contribute to salt resistance in rice.