Shear stress affects migration behavior of polymorphonuclear cells arrested on endothelium
Shear stress affects migration behavior of polymorphonuclear cells arrested on endothelium
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DOI：
10.1006/cimm.2000.1671
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发表时间：
2000-07-10
影响因子：
4.3
通讯作者：
Nagawa, H
中科院分区：
文献类型：
--
作者：
Kitayama, J;Hidemura, A;Nagawa, H
在剪切流存在下，用延时录像带监测多形核细胞（PMN）穿过TNF-α刺激的内皮细胞（HUVEC）单层的迁移。超过一半的中性粒细胞，逮捕HUVEC穿越内皮细胞连接在以下15分钟内。迁移的动力学明显快于静态条件下放置的中性粒细胞。一旦PMN爬入内皮下间隙，它们就表现出在HUVEC单层下的随机迁移。未迁移的PMN在HUVEC顶面沿顺流方向移动。抗β 1整合素单抗（4 B4）和RGD肽对迁移的抑制作用强于抗β 2整合素单抗（TS 1/18），几乎完全消除了迁移。当HUVEC在纤连蛋白或层粘连蛋白上培养时，抗α 5或α 6整合素单克隆抗体分别显著抑制HUVEC的迁移。我们的数据清楚地表明，切应力影响的迁移行为的中性粒细胞逮捕内皮细胞，并建议，结合内皮下细胞外基质通过β 1整合素是另一个重要的步骤，白细胞外渗。(C)北京大学出版社.
Polymorphonuclear cell (PMN) transmigration across the TNF-alpha-stimulated endothelial cell (HUVEC) monolayer in the presence of shear flow was monitored with time-lapse videotapes. More than half of the PMN that arrested on HUVEC transmigrated through endothelial cell junctions within the following 15 min. The kinetics of transmigration was significantly faster than that of PMN placed under static conditions. Once PMN crept into the subendothelial space, they showed random migration beneath the HUVEC monolayer. PMN that did not transmigrate moved on the apical surface of HUVEC in the direction of flow down stream. Anti-beta 1 integrin mAb (4B4) and RGD peptide inhibited the transmigration more effectively than anti-beta 2 integrin mAb (TS1/18) and almost totally abrogated transmigration. When HUVEC were cultured on fibronectin or laminin, the transmigration was significantly inhibited by anti-alpha 5 or alpha 6 integrin mAbs, respectively. Our data clearly indicate that shear stress affects the migration behavior of PMN arrested on endothelium and suggest that binding to subendothelial extracellular matrix via beta 1 integrins is another essential step in leukocyte extravasation. (C) 2000 Academic Press.