Long Open Reading Frame Transcripts Escape Nonsense-Mediated mRNA Decay in Yeast
Long Open Reading Frame Transcripts Escape Nonsense-Mediated mRNA Decay in Yeast
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DOI：
10.1016/j.celrep.2014.01.025
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发表时间：
2014-02-01
期刊：
影响因子：
8.8
通讯作者：
Saveanu, Cosmin
中科院分区：
文献类型：
--
作者：
Decourty, Laurence;Doyen, Antonia;Saveanu, Cosmin
无意义介导的信使核糖核酸衰变(NMD)使长3‘UTRs的真核转录本不稳定。为了研究其他转录特征是否影响NMD，我们产生了表达染色体来源的mRNAs的酵母菌株，这些mRNAs具有979个不同的启动子和开放阅读框(ORF)区域，并且具有相同的长、不稳定的3‘UTR。我们开发了一种基于条形码的DNA微阵列策略来比较具有或不具有NMD活性的菌株中每个报告基因的水平。编码区的大小对NMD效率有显著的负面影响。这种影响并不是被测试的3‘UTR所特有的，因为另外两个不同的NMD记者随着ORF长度的增加对NMD的敏感度降低。低效的NMD并不是由于UPF1与长的ORF转录本缺乏关联。总之，除了长的3‘非编码区外，短的翻译长度是酵母中NMD底物的一个重要特征。
Nonsense-mediated mRNA decay (NMD) destabilizes eukaryotic transcripts with long 3' UTRs. To investigate whether other transcript features affect NMD, we generated yeast strains expressing chromosomal-derived mRNAs with 979 different promoter and open reading frame (ORF) regions and with the same long, destabilizing 3' UTR. We developed a barcode-based DNA microarray strategy to compare the levels of each reporter mRNA in strains with or without active NMD. The size of the coding region had a significant negative effect on NMD efficiency. This effect was not specific to the tested 3' UTR because two other different NMD reporters became less sensitive to NMD when ORF length was increased. Inefficient NMD was not due to a lack of association of Upf1 to long ORF transcripts. In conclusion, in addition to a long 3' UTR, short translation length is an important feature of NMD substrates in yeast.