Cross-linking of neutrophil CD11b results in rapid cell surface expression of molecules required for antigen presentation and T-cell activation
Cross-linking of neutrophil CD11b results in rapid cell surface expression of molecules required for antigen presentation and T-cell activation
复制标题
DOI：
10.1111/j.1365-2567.2004.02114.x
复制
发表时间：
2005-03-01
期刊：
影响因子：
6.4
通讯作者：
Young, B
中科院分区：
文献类型：
--
作者：
Sandilands, GP;Ahmed, Z;Young, B
最近的研究表明，中性粒细胞可能在抗原呈递中发挥作用。为了支持这一假设，研究表明这些细胞似乎含有该功能所需分子的细胞质储存，即主要组织相容性复合物 II 类 (DR) 抗原、CD80 和 CD86。在这项研究中，我们考虑了将这些预先形成的分子易位到细胞表面上的机制，该机制不需要主动合成。 Mac-1 分子（CD18 + CD11b）的交联导致正常人外周血中性粒细胞表面 CD80、CD86 和 DR 抗原的快速细胞表面表达。与对照细胞相比，一个独特的中性粒细胞亚群（约 20%）似乎增大，并且发现在 CD11b 交联后细胞表面这些分子的表达水平显着升高。这些大细胞上CD80、CD86和DR抗原的表达水平与从相同供体获得的单核细胞表面上发现的表达水平相当，并且在某些情况下更高。此外，通过共聚焦激光显微镜和免疫电子显微镜显示这些细胞质分子位于分泌囊泡内。快速易位到细胞表面后，CD80 和 CD86 似乎共定位在大簇内，让人想起先前在常规抗原呈递细胞上发现的超分子抗原簇。因此，这些发现进一步支持了中性粒细胞可能在抗原呈递和/或 T 细胞激活中发挥作用的假设。
Recent studies suggest that neutrophils may play a role in antigen presentation. In support of this hypothesis it has been shown that these cells appear to contain cytoplasmic stores of molecules required for this function, i.e. major histocompatibility complex class II (DR) antigen, CD80 and CD86. In this study we have considered a mechanism for the translocation of these preformed molecules onto the cell surface which does not require active synthesis. Cross-linking of the Mac-1 molecule (CD18 + CD11b) was shown to result in rapid cell surface expression of CD80, CD86 and DR antigen on the surface of normal human peripheral blood neutrophils. A distinct subpopulation (approximately 20%) of neutrophils appeared to be enlarged and were found to express significantly elevated levels of these molecules on the cell surface following cross-linking of CD11b when compared with control cells. The level of expression of CD80, CD86 and DR antigen on these large cells was comparable to, and in some cases greater than, the levels found expressed on the surface of monocytes obtained from the same donors. In addition, these cytoplasmic molecules were shown by confocal laser microscopy and by immunoelectron microscopy to be located within secretory vesicles. Following rapid translocation onto the cell surface, CD80 and CD86 appeared to be colocalized within large clusters reminiscent of the supramolecular antigen clusters previously found on conventional antigen-presenting cells. These findings therefore lend further support for the hypothesis that neutrophils may have a role to play in antigen presentation and/or T-cell activation.