Detection of minor drug-resistant populations by parallel allele-specific sequencing
Detection of minor drug-resistant populations by parallel allele-specific sequencing
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DOI：
10.1038/nmeth995
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发表时间：
2007-02-01
期刊：
影响因子：
48
通讯作者：
Gao, Feng
中科院分区：
文献类型：
--
作者：
Cai, Fangping;Chen, Haifeng;Gao, Feng
我们开发了一种高灵敏度的平行等位基因特异性测序（PASS）检测方法，可同时分析大量病毒基因组，并检测出0.1-0.01%水平的轻微耐药人群。使用这种检测方法对人类免疫缺陷病毒（HIV）感染者的样本进行检测，我们成功地检测和定量了少数耐药病毒，并对多药耐药突变进行了连锁分析。这种检测方法可以作为一个有用的工具，研究艾滋病毒和其他感染性病原体的耐药性。
We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at similar to 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.