Multiple forms of myeloperoxidase from human neutrophilic granulocytes: evidence for differences in compartmentalization, enzymatic activity, and subunit structure.
Multiple forms of myeloperoxidase from human neutrophilic granulocytes: evidence for differences in compartmentalization, enzymatic activity, and subunit structure.
复制标题
来自人类中性粒细胞的多种形式的髓过氧化物酶：区室化、酶活性和亚基结构差异的证据。
DOI：
10.1016/0003-9861(83)90158-3
复制
发表时间：
1983
影响因子：
3.9
通讯作者：
KinkadeJr,JM
中科院分区：
文献类型：
--
作者：
Pember,SO;Shapira,R;KinkadeJr,JM
从单个供体获得的正常人嗜中性粒细胞的多种形式的髓过氧化物酶可以通过羧甲基（CM）-纤维素离子交换柱色谱法分解为三种形式（I，II和III），指定为使用线性盐梯度洗脱吸附的酶的顺序。通过洗涤剂（形式I）或高离子强度程序（形式II和III）选择性溶解酶的各个形式表明这些形式的酶被不同地区室化。所有三种形式通过优先提取、离子强度操纵以及离子交换和分子筛色谱法的组合进行纯化。纯化形式II和III对各种底物具有相似的比活性。I型对这些相同底物中的几种活性较低，最显著的是碘化物，比活性约为II型和III型的一半。所有的形式都有类似的光谱特性的一个typeaheme。三种形式的氨基酸组成相似，但在选定的残基（例如带电氨基酸）中发现显着差异。天然聚丙烯酰胺凝胶电泳解决了流动性的形式，这是一致的CM-纤维素上观察到的电荷异质性之间的小差异。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳数据与普遍接受的两条重链和两条轻链的亚基结构一致。所有三种形式都含有一个Mr 11，500的小分子量亚基。I型含有Mr 63，000的大亚基，而II型和III型含有Mr 57，500的相应亚基。我们的结论是，人类髓过氧化物酶的异质性伴随着细胞区室化，酶活性和亚基结构的差异。
Multiple forms of myeloperoxidase from normal human neutrophilic granulocytes obtained from a single donor can be resolved by carboxymethyl (CM)-cellulose ion-exchange column chromatography into three forms (I, II, and III) designated in order of elution of adsorbed enzyme using a linear salt gradient. Selective solubilization of individual forms of the enzyme by detergent (form I) or high-ionic-strength procedures (forms II and III) suggested that these forms of the enzyme were compartmentalized differently. All three forms were purified by a combination of preferential extraction, manipulation of ionic strength, and ion-exchange and molecular sieve chromatography. Purified forms II and III had similar specific activities for a variety of substrates. Form I was less active toward several of these same substrates, most notably iodide, with a specific activity about one-half that of forms II and III. All forms had similar spectral properties characteristic of a typeaheme. The amino acid compositions of the three forms were similar, yet significant differences were found in selected residues such as the charged amino acids. Native polyacrylamide gel electrophoresis resolved small differences in mobility between the forms which were consistent with the charge heterogeneity observed on CM-cellulose. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis data were consistent with the generally accepted subunit structure of two heavy chains and two light chains. All three forms contained a small-molecular-weight subunit ofMr11,500. Form I contained a large subunit ofMr63,000, while forms II and III contained a corresponding subunit ofMrapproximately 57,500. We conclude that heterogeneity of human myeloperoxidase is accompanied by differences in cellular Compartmentalization, enzymatic activity, and subunit structure.
登录
查看更多内容
人嗜酸性过氧化物酶：在杀菌活性中的作用。
影响因子：
20.3
作者：
R. Migler;L. Dechatelet;D. Bass
通讯作者：
D. Bass
羧甲基纤维素柱上核糖核酸酶的色谱分析。
DOI：
10.1016/s0021-9258(18)69753-4
发表时间：
1959
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Taborsky
通讯作者：
G. Taborsky
影响因子：
3.9
作者：
DESSER, RK;EVANS, WH;HIMMELHO.R
通讯作者：
HIMMELHO.R
包括抗甲状腺化合物在内的各种试剂对甲状腺过氧化物酶活性的影响。
DOI：
--
发表时间：
1963
期刊：
Journal of Biochemistry (Tokyo)
影响因子：
--
作者：
T. Hosoya
通讯作者：
T. Hosoya
人白细胞髓过氧化物酶的分离程序和一些特性。
DOI：
--
发表时间：
1978
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
A. Bakkenist;R. Wever;T. Vulsma;H. Plat;B. F. Gelder
通讯作者：
B. F. Gelder