Laxative effect of agarwood leaves and its mechanism
Laxative effect of agarwood leaves and its mechanism
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DOI：
10.1271/bbb.70361
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发表时间：
2008-02-01
影响因子：
1.6
通讯作者：
Iinuma, Munekazu
中科院分区：
文献类型：
--
作者：
Hara, Hideaki;Ise, Yasuaki;Iinuma, Munekazu
我们研究了沉香叶提取物的通便活性。通过计算小鼠的排便次数和粪便重量来测量通便活性，并口服给药。沉香叶的丙酮提取物和番泻叶（一种代表性的泻药）都增加了大便频率和重量，但甲醇提取物没有。丙酮提取物的通便作用比蒽醌类通便剂番泻叶温和，并且前者不会引起腹泻作为严重的副作用。我们鉴定了对丙酮提取物的通便作用有贡献的主要成分为芫花素5-O-β-樱草苷（化合物4）。化合物4增强回肠的自发运动并诱导回肠收缩。阿托品可抑制化合物4诱导的回肠收缩，但阿扎司琼不能抑制，这表明化合物4的作用是由乙酰胆碱受体介导的，而不是由5-羟色胺介导的。化合物4的通便机制可能部分涉及通过乙酰胆碱受体刺激肠运动。
We investigated the laxative activity of an extract of agarwood leaves from Aquilaria sinensis. The laxative activity was measured in mice by counting the stool frequency and stool weight, and the drugs were orally administered. An acetone extract of agarwood leaves and senna (a representative laxative drug) both increased the stool frequency and weight, but a methanol extract did not. The laxative effect of the acetone extract was milder than that of the anthraquinoid laxative, senna, and the former did not induce diarrhea as a severe side effect. We identified the main constituent contributing to the laxative effect of the acetone extract as genkwanin 5-O-beta-primeveroside (compound 4). Compound 4 strengthened the spontaneous motility and induced contraction in the ileum. This ileal contraction induced by compound 4 was inhibited by atropine, but not by azasetron, suggesting that the effect of compound 4 was mediated by acetylcholine receptors, and not by serotonin. The laxative mechanism for compound 4 may in part involve stimulation of intestinal motility via acetylcholine receptors.