Prime-boost vaccination using cysteine proteinases type I and II of Leishmania infantum confers protective immunity in murine visceral leishmaniasis
Prime-boost vaccination using cysteine proteinases type I and II of Leishmania infantum confers protective immunity in murine visceral leishmaniasis
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DOI：
10.1016/j.vaccine.2005.11.011
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发表时间：
2006-03-15
期刊：
影响因子：
5.5
通讯作者：
Nazgouee, F
中科院分区：
文献类型：
--
作者：
Rafati, S;Zahedifard, F;Nazgouee, F
用编码半胱氨酸蛋白酶的DNA混合物接种疫苗，先前已证明可对实验性皮肤利什曼病（CL）提供保护。在本研究中，我们在小鼠感染模型中测试了免疫对利什曼原虫的效力，使用了一种初始增强策略。用编码I型（cpb）和II型（cpa）半胱氨酸蛋白酶的质粒DNA混合免疫BALB/c小鼠，每隔3周免疫两次。DNA免疫后，除了CpG ODN和Montanide720作为佐剂外，还用rCPA/rCPB进行增强。免疫应答分析显示，接种主要引起抗原特异性IgG2a抗体，提示诱导Th1免疫应答。脾脏细胞因子产生的分析进一步证实了这一点：在所有时间点，接种组与rCPA和rCPB再刺激后诱导的ifn - γ /IL-5的比率始终显著高于两个对照组。(c) 2005 Elsevier Ltd版权所有。
Vaccination with a cocktail of DNA encoding cysteine proteinases has been previously shown to confer protection against experimental cutaneous leishmaniasis (CL). In the present study we test the efficacy of immunization against Leishmania infantum in a murine model of infection, using a prime-boost strategy. BALB/c mice were immunized twice, in a 3 weeks interval, with cocktail of plasmids DNA encoding type I (cpb) and II (cpa) cysteine proteinases. DNA immunization was then followed by a boost with rCPA/rCPB in addition to CpG ODN and Montanide720 as adjuvant.Analysis of the immune response showed that vaccination mainly elicited antigen-specific IgG2a antibodies, suggesting the induction of a Th1 immune response. This was further confirmed by the analysis of the splenic cytokine production: at all time points the ratio of IFN-gamma/IL-5 induced upon restimulation with rCPA and rCPB was always significantly higher in vaccinated group compared to both control groups. (c) 2005 Elsevier Ltd. All rights reserved.