Ribosomal DNA spacer genotypes of the Anopheles bancroftii group (Diptera: Culicidae) from Australia and Papua New Guinea
Ribosomal DNA spacer genotypes of the Anopheles bancroftii group (Diptera: Culicidae) from Australia and Papua New Guinea
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DOI：
10.1046/j.0962-1075.2001.00278.x
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发表时间：
2001-10-01
影响因子：
2.6
通讯作者：
Cooper, RD
中科院分区：
文献类型：
--
作者：
Beebe, NW;Maung, J;Cooper, RD
对采自澳大利亚北部和巴布亚新几内亚(PNG)的班克罗夫蒂按蚊(Anopheles Bancroftii)群体的蚊子进行了核糖体DNA ITS2序列的变异研究。将最初用于识别该组成员的翼缘形态与rDNA ITS2的限制性片段长度多态性分析(RFLP)和异源双链分析(HDA)所鉴定的基因型进行比较。该组成员可分为A、B、C和D四种RFLP基因型，部分基因型表现为翼缘多态。对群体内和群体间的ITS2进行的异源双链分析发现，A型包含两个地理上分离的ITS2序列：A1来自澳大利亚北领地，A2来自昆士兰和巴新西部省。基因B和C以及基因C和D被发现是共域的，并且似乎是独立进化的，这表明可能存在隐蔽的物种。基因C在基因组中包含两种ITS2序列类型。
Mosquitoes of the Anopheles bancroftii group collected from Northern Australia and Papua New Guinea (PNG) were investigated for sequence variation within the ribosomal DNA ITS2. Wing fringe morphology originally used to identify members of this group was compared to genotypes identified by restriction fragment length polymorphism analysis (RFLP) and heteroduplex analysis (HDA) of the rDNA ITS2. Members of this group separated into four RFLP genotypes (A, B, C and D) with some genotypes displaying wing fringe polymorphisms. Heteroduplex analysis of the ITS2 within and between populations identified genotype A as containing two geographically separate ITS2 sequences: A1 from the Northern Territory of Australia and A2 from Queensland and the Western Province of PNG. Genotypes B and C and genotypes C and D were found sympatric and appeared to be evolving independently suggesting the possibility of cryptic species. Genotype C contained two ITS2 sequence types within the genome.