MUTANTS OF SCHIZOSACCHAROMYCES-POMBE WHICH SPORULATE IN THE HAPLOID STATE
MUTANTS OF SCHIZOSACCHAROMYCES-POMBE WHICH SPORULATE IN THE HAPLOID STATE
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DOI：
10.1007/bf00332932
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
YAMAMOTO, M
中科院分区：
文献类型：
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作者：
IINO, Y;YAMAMOTO, M
mei1-102 的抑制突变体是交配型盒式基因 (mat2-P) 之一的突变，可阻止减数分裂的进展，在粟酒裂殖酵母中分离并表征。这些抑制突变赋予温度敏感性或冷敏感性。这些菌株的生长在限制性温度下停止并开始孢子形成，而不管减数分裂开始通常所需的其他条件如何，即它们在氮源和交配型纯合性存在的情况下形成孢子。它们最显着的特征是它们可以从单倍体状态产生孢子。这些突变体的单倍体通过遗传分析和细胞DNA含量的测量得到证实。这些突变体都是隐性的并且定义了单个基因 pat1。 pat1 基因定位非常靠近 II 号染色体的着丝粒。 mei5 中的减数分裂缺陷突变可以抑制 pat1 引起的温度敏感性，表明它们之间存在一定的相互作用。单倍体细胞产生的孢子活力较差，平均仅含有 1/2C DNA。
Suppressor mutants of mei1-102, a mutation in one of the mating type cassette genes (mat2-P) which blocks the progression into meiosis, were isolated and characterized in S. pombe. These suppressor mutations conferred either temperature-sensitivity or cold-sensitivity. The growth of these strains is halted and sporulation initiated at the restrictive temperatures, regardless of other conditions usually required for the initiation of meiosis i.e., they sporulate in the presence of a N source and mating type homozygosity. Their most striking feature is that they can sporulate from the haploid state. The haploidy of these mutants was confirmed by genetical analysis and by measurement of the DNA content of the cells. The mutants are all recessive and define a single gene pat1. The pat1 gene maps very close to the centromere of chromosome II. A meiosis defective mutation in mei5 can suppress the temperature-sensitivity caused by pat1, indicating some interaction between them. Spores produced from a haploid cell have poor viability and appear to contain only 1/2C DNA on average.