Stimulative effects of light and a temperature downshift on transcriptional expressions of developmentally regulated genes in the initial stages of fruiting-body formation of the basidiomycetous mushroom Lentinula edodes
Stimulative effects of light and a temperature downshift on transcriptional expressions of developmentally regulated genes in the initial stages of fruiting-body formation of the basidiomycetous mushroom Lentinula edodes
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DOI：
10.1111/j.1574-6968.2008.01364.x
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发表时间：
2008-12-01
影响因子：
2.1
通讯作者：
Shishido, Kazuo
中科院分区：
文献类型：
--
作者：
Nakazawa, Takehito;Miyazaki, Yasumasa;Shishido, Kazuo
通过定量反转录PCR实验，分析了光照和温度降温对香菇野生型菌株FMC2子实体形成初期多种发育调控基因转录表达的影响。结果表明，适当的光照条件和温度降移是高水平基因转录和子实体形成的必要条件。此前报道的L. edodes MIL-LEW-M13-1菌株不经低温处理也能形成子实体，光照增强了发育调控基因的表达。
Quantitative reverse transcription PCR experiments were done to analyze the effects of light and a temperature downshift on transcriptional expressions of a variety of developmentally regulated genes in the initial stages of fruiting-body formation of Lentinula edodes wild-type strain FMC2. It was revealed that both proper light conditions and a temperature downshift are required for high levels of transcriptions of the genes and the formation of fruiting bodies. In L. edodes MIL-LEW-M13-1 strain, which has been reported previously to be capable of forming fruiting bodies without a low-temperature treatment, the expressions of the developmentally regulated genes were enhanced by light exposure.