Stable isotope signatures confirm carbon and nitrogen gain through ectomycorrhizas in the ghost orchid Epipogium aphyllum Swartz.

Stable isotope signatures confirm carbon and nitrogen gain through ectomycorrhizas in the ghost orchid Epipogium aphyllum Swartz.
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稳定同位素特征证实鬼兰Epipogium aphyllum Swartz通过外生菌根获得碳和氮

DOI:
10.1111/j.1438-8677.2010.00369.x
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发表时间:
2011
期刊:
影响因子:
3.9
通讯作者:
Gebauer G.
Gebauer G.
中科院分区:
生物学2区
文献类型:
--
作者:
Liebel H.T;Gebauer G.

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Epipogium aphyllumis是一种罕见的欧亚无绿叶森林兰花,已知与形成外生菌根的真菌有关,而温暖潮湿气候的密切相关的兰花依赖于木材或垃圾分解真菌。我们进行了13 C和15 N稳定同位素天然丰度分析,以确定来自挪威中部的E.aphyllum的有机营养源。这些数据为兰花芽组织,在比较陪同的自养植物,文件C和N流从外生菌根真菌的兰花。从兰花根皮层的真菌群体中提取的DNA数据证实了Inocybe和Hebeloma的存在,这两种真菌都形成了外生菌根。利用E.aphyllum的13 C和15 N富集因子计算了与外生菌根真菌共生的异养真菌的总平均富集因子(ε 13 C ± 1 SD为7.2 ± 1.6‰,ε 15 N ± 1 SD为12.8 ± 3.9‰)。          这些可以用来估计真菌的贡献,有机养分吸收的部分mycoheterotrophic植物,完全mycoheterotrophic植物是缺乏。兰花组织中的氮浓度异常高,显着高于伴随的自养叶样品。这可能是由于外生菌根真菌对无叶卫矛氮素的吸收造成的。我们表明,E. aphyllum是一种表寄生真菌异养兰花,依赖于外生菌根Inocybea和Hebelomato,通过真菌将真菌异养植物与森林树木连接起来的三方系统获得C和N。
Epipogium aphyllumis a rare Eurasian achlorophyllous forest orchid known to associate with fungi that form ectomycorrhizas, while closely related orchids of warm humid climates depend on wood‐ or litter‐decomposer fungi. We conducted13C and15N stable isotope natural abundance analyses to identify the organic nutrient source ofE.aphyllumfrom Central Norway. These data for orchid shoot tissues, in comparison to accompanying autotrophic plants, document C and N flow from ectomycorrhizal fungi to the orchid. DNA data from fungal pelotons in the orchid root cortex confirm the presence ofInocybeandHebeloma, which are both fungi that form ectomycorrhizas. The enrichment factors for13C and15N ofE. aphyllumare used to calculate a new overall average enrichment factor for mycoheterotrophic plants living in association with ectomycorrhizal fungi (ε13C ± 1 SD of 7.2 ± 1.6‰ and ε15N ± 1 SD of 12.8 ± 3.9‰). These can be used to estimate the fungal contribution to organic nutrient uptake by partially mycoheterotrophic plants where fully mycoheterotrophic plants are lacking. N concentrations in orchid tissue were unusually high and significantly higher than in accompanying autotrophic leaf samples. This may be caused by N gain ofE.aphyllumfrom obligate ectomycorrhizal fungi. We show thatE. aphyllumis an epiparasitic mycoheterotrophic orchid that depends on ectomycorrhizalInocybeandHebelomato obtain C and N through a tripartite system linking mycoheterotrophic plants through fungi with forest trees.
热解菌、杜鹃花科真菌和兰花中的氮和碳增益具有广泛的地理和生态分布。
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稳定天然氮、碳和硫同位素生态系统循环的生物和非生物控制
DOI: --
发表时间: 2000
期刊:
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