Separation of bovine serum albumin (BSA) using γ-Al2O3-clay composite ultrafiltration membrane
Separation of bovine serum albumin (BSA) using γ-Al2O3-clay composite ultrafiltration membrane
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DOI：
10.1002/jctb.2326
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发表时间：
2010-04-01
影响因子：
3.4
通讯作者：
Pugazhenthi, G.
中科院分区：
文献类型：
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作者：
Monash, P.;Majhi, Abhijit;Pugazhenthi, G.
背景技术背景：陶瓷膜由于其优良的上级化学、机械和热性能，在生物产品的分离和纯化方面比聚合物膜受到更多的关注。市售的陶瓷膜太贵。这可以通过使用低成本原材料制造膜来克服。本工作的目的是制备一种低成本的γ-Al 2 O3-粘土复合膜，并评估其分离牛血清白蛋白（BSA）作为pH值，进料浓度和施加压力的函数的潜力。为了实现这一点，使用低成本的粘土混合物代替非常昂贵的氧化铝、氧化锆和二氧化钛材料来制备膜支撑体。结果：γ-Al_2O_3-粘土复合膜的孔径分布为5.4- 13.6nm。该膜使用窄粒度分布和平均粒度30.9 nm的稳定勃姆石溶胶制备。扫描电子显微镜证实，γ-Al 2 O3-粘土复合膜的表面是无缺陷的。复合膜的纯水透过率为2.357 × 10 ~（-7）m ~（-3）m ~（-2）s ~（-1）kPa ~（-1）。BSA蛋白的最大截留率为95%。据观察，膜的分离性能在通量和排斥强烈依赖于蛋白质和带电membranes.CONCLUSION之间的静电相互作用：连续制备的γ-Al 2 O3-粘土复合膜被证明具有良好的潜力，BSA的分离与高产率，可以作为一种低成本的替代昂贵的陶瓷膜。(C)2009年化学工业学会
BACKGROUND: Ceramic membranes have received more attention than polymeric membranes for the separation and purification of bio-products owing to their superior chemical, mechanical and thermal properties. Commercially available ceramic membranes are too expensive. This could be overcome by fabricating membranes using low-cost raw materials. The aim of this work is to fabricate a low-cost gamma-Al2O3-clay composite membrane and evaluate its potential for the separation of bovine serum albumin (BSA) as a function of pH, feed concentration and applied pressure. To achieve this, the membrane support is prepared using low-cost clay mixtures instead of very expensive alumina, zirconia and titania materials. The cost of the membrane can be further reduced by preparing a gamma-alumina surface layer on the clay support using boehmite sol synthesized from inexpensive aluminium chloride instead of expensive aluminium alkoxide using a dip-coating technique.RESULTS: The pore size distribution of the gamma-Al2O3-clay composite membrane varied from 5.4-13.6 nm. The membrane was prepared using stable boehmite sol of narrow particle size distribution and mean particle size 30.9 nm. Scanning electron microscopy confirmed that the surface of the gamma-Al2O3-clay composite membrane is defect-free. The pure water permeability of the support and the composite membrane were found to be 4.838 x 10(-6) and 2.357 x 10(-7) m(3) m(-2) s(-1) kPa(-1), respectively. The maximum rejection of BSA protein was found to be 95%. It was observed that the separation performance of the membrane in terms of flux and rejection strongly depends on the electrostatic interaction between the protein and charged membrane.CONCLUSION: The successively prepared gamma-Al2O3-clay composite membrane proved to possess good potential for the separation of BSA with high yield and could be employed as a low cost alternate to expensive ceramic membranes. (C) 2009 Society of Chemical Industry