The parasitic dinoflagellate Hematodinium infects multiple crustaceans in the polyculture systems of Shandong Province, China
The parasitic dinoflagellate Hematodinium infects multiple crustaceans in the polyculture systems of Shandong Province, China
复制标题
寄生甲藻Hematodinium感染中国山东省混养系统中的多种甲壳类动物
DOI：
10.1016/j.jip.2020.107523
复制
发表时间：
2021-01-01
影响因子：
3.4
通讯作者：
Li, Caiwen
中科院分区：
文献类型：
--
作者：
Li, Meng;Huang, Qian;Li, Caiwen
寄生鞭毛藻属（Hematodinium）已经影响了世界范围内野生和养殖的商业甲壳类动物种群。近十年来，山东半岛混养池养殖的中华梭子蟹（Portunus trituberculatus）发生了动物血虫病，造成大量死亡，而混养池系统中该寄生虫的来源和传播尚不清楚。2018年4 - 12月，在山东省青岛市血液病高流行区混养池系统中采集了2034只螃蟹和108只对虾。其中，6种蟹类188只，包括4种新寄主（弓形蟹、青霉蟹、武汉蟹和日本巨眼蟹）。单斑对虾未发现感染。进一步的系统发育分析表明，感染6只蟹宿主的分离物与中国报道的其他分离物共同构成perezi血液病基因型II型。该寄生虫对养殖的三管梭子蟹（Portunus trituberculatus）和优势野生蟹（Helice tientsinensis）的传染性更强，尽管它是一种寄主通才型病原体。中华绒螯蟹的佩氏血液病感染率高于其他野生蟹类，与养殖的三瘤梭子蟹呈显著正相关。结果表明，野生蟹类，尤其是天津海蟹，是与血球病在混养池系统中传播和传播密切相关的重要交替宿主。
The parasitic dinoflagellates of the Hematodinium genus have impacted wild and cultured stocks of commercial crustaceans worldwide. In the past decade, outbreaks of Hematodinium epizootics resulted in substantial mortalities in cultured Chinese swimming crabs Portunus trituberculatus in the polyculture ponds located in Shandong Peninsula, whereas the source and transmission of the parasite in the polyculture pond system remains to be determined. During April to December of 2018, 2034 crabs and 108 shrimps were collected from the polyculture pond systems in the highly endemic area of Hematodinium diseases in Qingdao, Shandong Province. Among those, 188 individuals of the 6 crab species were infected by the parasite, including 4 novel host species (Uca arcuate, Hemigrapsus penicillatus, Helice wuana and Macrophthalmus japonicas). No infection was identified in Penaeus monodon. Further phylogenetic analyses indicated that the Hematodinium isolate infecting the six crab hosts, together with other isolates reported from China, composed the genotype II of Hematodinium perezi. The parasite was more infectious to cultured Portunus trituberculatus and the dominant wild crab Helice tientsinensis dwelling in the waterways connecting to the polyculture ponds, even though it was found to be a host generalist pathogen. The prevalence of Hematodinium perezi infection in Helice tientsinensis was higher than that of other wild crabs and showed significant positive correlation with that of the cultured Portunus trituberculatus. The results indicated that the wild crabs, particularly Helice tientsinensis, were the important alternate hosts closely involved in transmission and spreading of the Hematodinium disease in the polyculture pond systems.