1,25-dihydroxyvitamin D-3, transforming growth factor beta 1, calcium, and ultraviolet B radiation induce apoptosis in cultured human keratinocytes
1,25-dihydroxyvitamin D-3, transforming growth factor beta 1, calcium, and ultraviolet B radiation induce apoptosis in cultured human keratinocytes
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DOI：
10.1111/1523-1747.ep12335756
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发表时间：
1997-09-01
影响因子：
6.5
通讯作者：
Pincelli, C
中科院分区：
文献类型：
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作者：
Benassi, L;Ottani, D;Pincelli, C
细胞凋亡是一种自我定向自杀的细胞过程，在不断更新的组织的形态发生和维持稳态中发挥着关键作用，目前，细胞凋亡主要通过片段DNA的凝胶电泳和典型的超微结构特征（如细胞皱缩和染色质浓缩）来检测，最近，开发了一种原位技术，其允许检测细胞中的凋亡过程和定量细胞群中的凋亡，我们应用这项技术来评估在基础条件下培养的正常人角质形成细胞中的细胞凋亡过程，以及在用推测但从未被证明可诱导这些细胞凋亡的因子和试剂刺激后，在用1，25-二羟维生素D-3[1，25（OH）（2）D-3]、转化生长因子β 1（TGF β 1）、钙、UVB或肿瘤坏死因子α（TNF α）刺激后分析细胞凋亡。而UVB和钙分别在6和24 h是良好的促凋亡刺激，维生素D衍生物和TGF β 1在培养5和6 d后诱导细胞凋亡。最后，TUNEL技术显示，即使在未处理的角质形成细胞中，凋亡细胞的数量从24小时到144小时也略有增加（5-10%）。我们的研究表明，通常参与细胞生长和分化调节的因子也可以控制凋亡。
Apoptosis is a cellular process of self-directed suicide that plays a key role during morphogenesis and in the maintenance of homeostasis in continuously renewing tissues, Currently, apoptosis is detected mainly by gel electrophoresis of fragmented DNA and by typical ultrastructural features such as cell shrinkage and chromatin condensation, Recently, an in situ technique was developed that allows the detection of the apoptotic process in cells and the quantitation of apoptosis in cell populations, We applied this technique to evaluate the apoptotic process in cultured normal human keratinocytes under basic conditions and after stimulation with factors and agents that are presumed but have never been proved to induce apoptosis in these cells, Apoptosis was analyzed after stimulation with 1,25-dihydroxyvitamin D-3[1,25(OH)(2)D-3], transforming growth factor beta 1 (TGF beta 1), calcium, UVB, or tumor necrosis factor alpha (TNF alpha), All these factors except TNF alpha induced apoptosis in human keratinocytes. Whereas UVB and calcium were good apoptogenic stimuli at 6 and 24 h, respectively, the vitamin D derivative and TGF beta 1 induced apoptosis after 5 and 6 d in culture, Apoptosis was also established by DNA fragmentation and electron microscopy. Finally, TUNEL technique showed that the number of apoptotic cells increases slightly (5-10%) from 24 to 144 h even in untreated keratinocytes, Our studies indicate that factors normally involved in the regulation of cell growth and differentiation can also control apoptosis.