Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations
Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations
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DOI：
10.1111/j.1399-302x.2009.00511.x
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发表时间：
2009-06-01
影响因子：
--
通讯作者：
Shizukuishi, S.
中科院分区：
文献类型：
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作者：
Kuboniwa, M.;Amano, A.;Shizukuishi, S.
[10]张文，张文.牙龈卟啉单胞菌的同型生物膜结构受到FimA类型变异的影响。口腔微生物免疫学2009：24：260-263。(C)牙龈卟啉单胞菌（Porphyromonas gingivalis）是一种牙周病病原体，其长菌毛（FimA）根据fimA基因的多样性分为六种基因型（I-V和Ib型）。FimA的变化以前被证明是与成人牙周炎在一般人群中的发病和发展，而FimA最近被发现是初始生物膜形成的关键介质。然而，目前尚不清楚FimA变异是否对生物膜特征有影响。在此，我们比较了具有不同FimA类型的牙龈卟啉单胞菌菌株形成的同型生物膜的特征结构。生物膜形成在磷酸盐缓冲盐水中的唾液涂覆的玻璃底威尔斯上，并使用共聚焦激光扫描显微镜分析其结构。此外，生物膜的生物体积的定量与三维荧光光度法。生物膜的结构由6个代表性的FimA型菌株形成的明显不同。I型和Ib型牙龈卟啉单胞菌形成的生物膜具有致密的基底单层和分散的小菌落，而II型、III型和IV型菌株形成的生物膜具有明显丰富的生物膜，充满了广泛聚集的高菌落，并且它们的生物体积显著大于I型和Ib型。这些特征被证实是密切相关的FimA类型的检测，利用FimA取代的突变体从I型到II型和那些从II型到I型。我们的研究结果表明，FimA的变化有影响的生物膜形成的牙龈卟啉单胞菌的结构，这可能是牙周炎的发病机制中的一个重要因素。
Kuboniwa M, Amano A, Inaba H, Hashino E, Shizukuishi S. Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations.Oral Microbiol Immunol 2009: 24: 260-263. (C) 2009 John Wiley & Sons A/S.Porphyromonas gingivalis is a periodontal pathogen whose long fimbriae (FimA) are classified into six genotypes (types I-V and Ib) based on the diversity of the fimA genes. FimA variations were previously shown to be related to the onset and development of adult periodontitis in a general population, while FimA were recently found to be critical mediators of initial biofilm formation. However, it is unclear if FimA variations have effects on biofilm features. Here, we compare the characteristic structures of homotypic biofilms developed by P. gingivalis strains with different FimA types.Biofilms were formed on saliva-coated glass bottom wells in phosphate-buffered saline and their structures were analysed using confocal laser scanning microscopy. Furthermore, the biovolumes of the biofilms were quantified with a three-dimensional fluorophotometric method.Biofilm structures formed by the six representative FimA-type strains apparently differed. Type I and Ib P. gingivalis formed biofilms with a dense basal monolayer and dispersed microcolonies, whereas those formed by types II, III and IV strains had markedly luxuriant biofilms filled with widely clumped and tall colonies, and their biovolumes were significantly greater than those of types I and Ib. These characteristic features were confirmed to be closely related to FimA type in assays that utilized fimA-substituted mutants from type I to II and those from type II to I.Our results suggest that FimA variations have effects on the structures of biofilms formed by P. gingivalis, which may be an important factor in the pathogenesis of periodontitis.