Chromosome dynamics in the yeast interphase nucleus
Chromosome dynamics in the yeast interphase nucleus
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DOI：
10.1126/science.1065366
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发表时间：
2001-12-07
期刊：
影响因子：
56.9
通讯作者：
Gasser, SM
中科院分区：
文献类型：
--
作者：
Heun, P;Laroche, T;Gasser, SM
关于间期核中染色体的动力学知之甚少。通过标记四个染色体区域与绿色荧光蛋白融合乳糖阻遏物，我们监测的运动和亚核位置的酵母基因组中的特定位点，在短时间间隔采样。我们发现，早期和晚期复制起点在G（1）相中是高度移动的，经常以能量依赖的方式以0.5微米/10秒或更快的速度移动。在G（1）中检测到的染色质的快速扩散运动通过依赖于活性DNA复制的机制在S期受到限制。相比之下，端粒和着丝粒在G（1）和S期都对染色质运动提供了不依赖复制的约束。
Little is known about the dynamics of chromosomes in interphase nuclei. By tagging four chromosomal regions with a green fluorescent protein fusion to lac repressor, we monitored the movement and subnuclear position of specific sites in the yeast genome, sampling at short time intervals. We found that early and late origins of replication are highly mobile in G(1) phase, frequently moving at or faster than 0.5 micrometers/10 seconds, in an energy-dependent fashion. The rapid diffusive movement of chromatin detected in G(1) becomes constrained in S phase through a mechanism dependent on active DNA replication. In contrast, telomeres and centromeres provide replication-independent constraint on chromatin movement in both G(1) and S phases.