The association of ribosomal protein L18 with Newcastle disease virus matrix protein enhances viral translation and replication
The association of ribosomal protein L18 with Newcastle disease virus matrix protein enhances viral translation and replication
复制标题
核糖体蛋白 L18 与新城疫病毒基质蛋白的关联增强病毒翻译和复制
DOI：
10.1080/03079457.2021.2013435
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发表时间：
2022-01-25
期刊：
影响因子：
2.8
通讯作者：
Han, Yifan
中科院分区：
文献类型：
--
作者：
Duan, Zhiqiang;Tang, Hong;Han, Yifan
大量研究表明，病毒可以利用或操纵核糖体蛋白来实现病毒蛋白的生物合成和复制。在我们最近对病毒感染细胞的蛋白质组学分析中，我们发现核糖体蛋白L18（RPL 18）是最高上调的差异表达蛋白，沿着在纽卡斯尔病毒（NDV）感染后期表达增加的病毒蛋白。然而，RPL 18与病毒蛋白质生物合成和NDV复制的关联仍不清楚。在本研究中，我们发现RPL 18的表达和转录水平在NDV感染早期降低，但在NDV感染后期增加。此外，细胞质NDV基质（M）蛋白的存在是导致病毒感染细胞和质粒转染细胞中RPL 18表达增加的原因。此外，细胞质M蛋白以剂量依赖性方式增加RPL 18的表达，即使它们彼此不相互作用。此外，siRNA介导的RPL 18敲低或RPL 18过表达通过减少或增加病毒蛋白翻译而不是病毒RNA合成和转录显著减少或增强NDV复制。综上所述，这些结果表明，RPL 18的表达增加可能与M蛋白的物理聚集在一起有关，这反过来又促进了病毒蛋白的生物合成和NDV的复制。
Numerous studies have shown that viruses can utilize or manipulate ribosomal proteins to achieve viral protein biosynthesis and replication. In our recent studies using proteomics analysis of virus-infected cells, we found that ribosomal protein L18 (RPL18) was the highest up-regulated differentially expressed protein, along with the increasingly expressed viral proteins later in Newcastle disease virus (NDV) infection. However, the association of RPL18 with viral protein biosynthesis and NDV replication remains unclear. In this study, we found that the expression and transcription levels of RPL18 was reduced early in NDV infection but increased later in NDV infection. In addition, the presence of cytoplasmic NDV matrix (M) protein was responsible for the increased expression of RPL18 in both virus-infected cells and plasmid-transfected cells. Moreover, cytoplasmic M protein increased RPL18 expression in a dose-dependent manner, even though they did not interact with each other. Furthermore, siRNA-mediated knockdown of RPL18 or overexpression of RPL18 dramatically reduced or enhanced NDV replication by decreasing or increasing viral protein translation rather than viral RNA synthesis and transcription. Taken together, these results suggested that the increased expression of RPL18 might be associated with the physical clumping together of the M protein, which in turn promoted viral protein biosynthesis and NDV replication.