Immunologic and molecular characterizations of T cell-derived T cell activating factor.
Immunologic and molecular characterizations of T cell-derived T cell activating factor.
复制标题
T 细胞衍生的 T 细胞激活因子的免疫学和分子特征。
作者：
M. Okada;N. Yoshimura;Y. Ichimori;S. Kishimoto;S. Nakai;N. Nishino;T. Kishimoto
PMA刺激的人T细胞杂交系（24A）的几个亚克隆的培养上清在ConA刺激的小鼠胸腺细胞增殖中显示出共刺激活性，但不显示任何IL 2活性。一些亚克隆没有显示出共刺激活性，即使当刺激PMA，排除了遗留效应的可能性。在培养上清中发现的因子增加了用次适浓度PHA刺激的正常T细胞中IL 2的产生。该因子还诱导T杂交克隆T-394.1产生IL 2，当后者用次优浓度的有丝分裂原刺激时，表明该T细胞衍生因子对有丝分裂原刺激的T细胞诱导IL 2产生的直接影响。该因子还诱导产生其他淋巴因子，如BCDF和IFN-γ。北方印迹分析表明，该因子诱导IL 2和IL 2受体mRNA的增加。这些结果表明T细胞能分泌具有IL-1样活性的因子。然而，北方印迹分析显示，来自T杂交克隆的mRNA不与来自人单核细胞的IL 1（β）的cDNA交叉反应。
Culture supernatants from several subclones of a human T hybrid line (24A) stimulated with PMA showed co-stimulatory activity in the proliferation of Con A-stimulated murine thymocytes, but did not show any IL 2 activity. Some subclones did not show co-stimulatory activity even when stimulated with PMA, excluding the possibility of a carry-over effect. The factor found in the culture supernatants increased IL 2 production in normal T cells stimulated with a suboptimal concentration of PHA. The factor also induced IL 2 production in a T hybrid clone, T-394.1, when the latter was stimulated with a suboptimal concentration of mitogens, indicating a direct effect by this T cell-derived factor on mitogen-stimulated T cells inducing IL 2 production. This factor also induced the generation of other lymphokines such as BCDF and IFN-gamma. Northern blot analysis showed that the factor induced an increase in mRNA for IL 2 as well as IL 2 receptor. These results indicated that T cells could secrete a factor with IL 1-like activity. However, Northern blot analysis showed that mRNA from a T hybrid clone does not cross-react with cDNA for IL 1 (beta) derived from human monocytes.